Vinothiya Kolanji, Ashokkumar Natarajan
Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalai Nagar 608 002 Tamil Nadu, India.
Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalai Nagar 608 002 Tamil Nadu, India.
Biomed Pharmacother. 2017 Mar;87:640-652. doi: 10.1016/j.biopha.2016.12.134. Epub 2017 Jan 11.
The worldwide incidence of diabetes has increased dramatically along with widespread lifestyle and dietary changes. Diets high in fat are strongly associated with the development of obesity and can induce insulin resistance in humans and animals. It is clear that obesity constitutes a risk factor for contributing to the development of type 2 diabetes. In the present study, we investigated the therapeutic potential action of vanillic acid on diabetes associated complications using a rat model. Rats were made diabetic hypertensive by high fat diet (HFD) for 20 weeks and were treated with vanillic acid (50mg/kg bw) for last 8 weeks. The effects of vanillic acid on glucose, plasma insulin, systolic and diastolic blood pressure, thiobarbituric acid reactive substances (TBARS), hydroperoxides as a lipid peroxidation marker, and the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), reduced glutathione (GSH), vitamin C and vitamin E as an antioxidant marker, AST and ALT as a liver function marker, urea, uric acid and creatinine as a kidney function marker were investigated. Histopathology of liver and kidney was also investigated as part of the pathology of diabetes. Treatment of diabetic rats with oral administration of vanillic acid at a dose of 50mgkg/body weight for 8 weeks resulted in a significant decrease in fasting plasma glucose, insulin and blood pressure levels in comparison with diabetic control group. The antioxidant activities were significantly increased and the levels of lipid peroxidation markers were significantly decreased in diabetic hypertensive rats treated with vanillic acid. These results suggest that vanillic acid offer a modulatory effect on control of diabetic hypertension by reduction of blood glucose, insulin and blood pressure, combating oxidative stress by activation of tissue antioxidants.
随着全球范围内生活方式和饮食习惯的广泛改变，糖尿病的发病率急剧上升。高脂肪饮食与肥胖的发生密切相关，并可在人和动物中诱发胰岛素抵抗。显然，肥胖是导致2型糖尿病发生的一个危险因素。在本研究中，我们使用大鼠模型研究了香草酸对糖尿病相关并发症的治疗潜在作用。通过高脂饮食（HFD）使大鼠患糖尿病高血压20周，并在最后8周用香草酸（50mg/kg体重）进行治疗。研究了香草酸对血糖、血浆胰岛素、收缩压和舒张压、硫代巴比妥酸反应物质（TBARS）、作为脂质过氧化标志物的氢过氧化物，以及作为抗氧化标志物的超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPx）、还原型谷胱甘肽（GSH）、维生素C和维生素E的活性，作为肝功能标志物的AST和ALT，作为肾功能标志物的尿素、尿酸和肌酐的影响。作为糖尿病病理学的一部分，还研究了肝脏和肾脏的组织病理学。与糖尿病对照组相比，以50mg/kg体重的剂量口服香草酸治疗糖尿病大鼠8周，可使空腹血糖、胰岛素和血压水平显著降低。在用香草酸治疗的糖尿病高血压大鼠中，抗氧化活性显著增加，脂质过氧化标志物水平显著降低。这些结果表明，香草酸通过降低血糖、胰岛素和血压，激活组织抗氧化剂对抗氧化应激，对糖尿病高血压的控制具有调节作用。
