Metabolic Syndrome Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran; Student Research Committee, Department of Nutrition, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran; International UNESCO Center for Health Related Basic Sciences and Human Nutrition, Department of Nutrition, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Department of Medical Genetics, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Arch Med Res. 2020 Apr;51(3):261-267. doi: 10.1016/j.arcmed.2020.02.006. Epub 2020 Feb 26.
Several chronic diseases are mediated by oxidative stress. Oxidative stress affects cell morphology and function and is associated with alterations in the serum protein component. In the current study, we analyzed four individual prognostic factors associating with serum Pro-Oxidant-Antioxidant Balance (PAB): neutrophil to lymphocyte ratio (NLR), Vitamin D, anti-heat shock protein 27 (anti-hsp27) antibody titer, and red blood cell distribution width (RDW) to evaluate them as the potential prognostic markers. In the current study, we attempted to investigate the relationship between serum PAB, RDW, NLR, serum vitamin D and anti-hsp27 concentration.
A total of 852 participants (438 males and 414 females) aged 47.64 ± 7.77 years were recruited in a cross-sectional study based on the Mashhad stroke and heart atherosclerotic disorders (MASHAD) cohort study data. Hematological parameters, and vitamin D, PAB and anti-hsp27 antibody titers were measured using the Sysmex auto analyzer system and enzyme-linked immune sorbent assay (ELISA), respectively.
The results showed a significant correlation between Vitamin D and anti-hsp27 antibody titers (r = -0.13 and p <0.001) as well as between RDW and serum PAB (r = 0.120 and p <0.001). Moreover, we found that serum PAB was positively associated with serum anti hsp27 antibody titers. The results showed increasing 1 unit of serum vitamin D can cause 3% decreases in anti hsp 27 values (OR = 0.97; CI 95% (0.96-0.99); p = 0.004). While this association was not significant for RDW, NLR and PAB (p >0.05) we found a significant association between serum PAB and serum anti hsp-27 antibody titers. Subjects with PAB levels 36.31-82.63 had a higher risk (1.83 fold) of having an increased anti-hsp27 antibody titers in comparison to the reference group (PAB level <36.31) (OR = 1.83 (95% CI = 1.33-2.52), p <0.001).
The present study shows that serum vitamin D can be associated with reduction in inflammatory status probably by decreasing levels of serum anti-hsp27 antibody titers, reduction in oxidative stress and therefore may reduce the risk of cardiovascular diseases. Anti-hsp27 antibody titers are associated with oxidative stress through the serum PAB, therefore these factors may be of prognostic values in detecting oxidative stress and risk of atherosclerosis. The evaluation of these factors in a larger population may help further confirm these findings.
几种慢性疾病是由氧化应激介导的。氧化应激影响细胞形态和功能，并与血清蛋白成分的改变有关。在目前的研究中，我们分析了与血清 Pro-Oxidant-Antioxidant Balance（PAB）相关的四个个体预后因素：中性粒细胞与淋巴细胞比值（NLR）、维生素 D、抗热休克蛋白 27（anti-hsp27）抗体滴度和红细胞分布宽度（RDW），以评估它们作为潜在的预后标志物。在目前的研究中，我们试图探讨血清 PAB、RDW、NLR、血清维生素 D 和抗 hsp27 浓度之间的关系。
在基于马什哈德中风和心脏动脉粥样硬化障碍（MASHAD）队列研究数据的横断面研究中，共招募了 852 名年龄为 47.64±7.77 岁的参与者（男性 438 名，女性 414 名）。使用 Sysmex 自动分析仪系统和酶联免疫吸附测定（ELISA）分别测量血液学参数以及维生素 D、PAB 和抗 hsp27 抗体滴度。
结果表明，维生素 D 与抗 hsp27 抗体滴度之间存在显著相关性（r=-0.13，p<0.001），RDW 与血清 PAB 之间也存在显著相关性（r=0.120，p<0.001）。此外，我们发现血清 PAB 与血清抗 hsp27 抗体滴度呈正相关。结果表明，血清维生素 D 增加 1 个单位可导致抗 hsp27 值降低 3%（OR=0.97；95%CI（0.96-0.99）；p=0.004）。虽然这种关联对于 RDW、NLR 和 PAB 并不显著（p>0.05），但我们发现血清 PAB 与血清抗 hsp-27 抗体滴度之间存在显著关联。与参考组（PAB 水平<36.31）相比，PAB 水平在 36.31-82.63 之间的患者发生抗 hsp27 抗体滴度升高的风险更高（1.83 倍）（OR=1.83（95%CI=1.33-2.52），p<0.001）。
本研究表明，血清维生素 D 可能通过降低血清抗 hsp27 抗体滴度、降低氧化应激来减轻炎症状态，从而降低心血管疾病的风险。抗 hsp27 抗体滴度通过血清 PAB 与氧化应激相关，因此这些因素在检测氧化应激和动脉粥样硬化风险方面可能具有预后价值。在更大的人群中评估这些因素可能有助于进一步证实这些发现。
