Department of Technology, State University of Feira de Santana, Feira de Santana, BA, 44.036-900, Brazil.
Department of Biotechnology, Lorena Engineering School, University of São Paulo, Lorena, SP, 12602-810, Brazil.
Appl Biochem Biotechnol. 2020 May;191(1):212-225. doi: 10.1007/s12010-020-03267-0. Epub 2020 Feb 28.
This work evaluated the effect of the cowpea (Vigna unguiculata L. Walp) extract concentration on mead production. Fermentations were carried out in 500-mL Erlenmeyer flasks containing 250 mL of honey wort (30 °Brix), supplemented with 1 g L of ammonium sulfate and 0.1 g L of magnesium chloride and the cowpea extract (5 and 30 g L), inoculated with 10 cells mL, and incubated at 30 °C for 240 h. Higher cell growth ((cells mL): 11.1 × 10, 11.3 × 10, and 19.6 × 10; substrate consumption (%): 86.0, 90.0, and 85.0) and ethanol production ((v v %): 15.0, 15.5, and 14.1) for yeasts Safbrew T-58, Premier Blanc, and Premier Cuvée, respectively, were obtained with 30 g L of bean extract. S. bayanus Premier Blanc had the best metabolic activity with lowest glycerol production (8.5 g L) and highest ethanol volumetric yields (0.51-1.52 h) after 48 h of fermentation.
这项工作评估了豇豆（Vigna unguiculata L. Walp）提取物浓度对蜂蜜酒生产的影响。在 500 毫升的 Erlenmeyer 锥形瓶中进行发酵，每个锥形瓶中含有 250 毫升蜂蜜麦芽汁（30 波美度），添加 1 g L 的硫酸铵和 0.1 g L 的氯化镁以及豇豆提取物（5 和 30 g L），接种 10 个细胞 mL，并在 30°C 下孵育 240 h。酵母 Safbrew T-58、Premier Blanc 和 Premier Cuvée 的细胞生长更高（（细胞 mL）：11.1×10、11.3×10 和 19.6×10；基质消耗（%）：86.0、90.0 和 85.0）和乙醇产量更高（（v v %）：15.0、15.5 和 14.1），豇豆提取物浓度为 30 g L。在发酵 48 h 后，Saccharomyces bayanus Premier Blanc 的代谢活性最佳，甘油产量最低（8.5 g L），乙醇体积产率最高（0.51-1.52 h）。
