Department of Ophthalmology, Beijing Key Laboratory of Restoration of Damaged Ocular Nerve, Peking University Third Hospital, Beijing, China.
Medical Research Center, Peking University Third Hospital, Beijing, China.
Lasers Med Sci. 2020 Aug;35(6):1299-1305. doi: 10.1007/s10103-019-02911-4. Epub 2020 Feb 29.
The purpose was to investigate the effect of daylight-intensity blue light on F. solani isolated from the cornea of patients with fungal keratitis. Spore suspensions of 5 F. solani strains (one standard strain and 4 clinical corneal isolates) were prepared in 6-well plates. Blue light groups were irradiated by a light-emitting diode (LED) device with a peak wavelength of 454 nm at 0.5 mW/cm for 0 to 48 h, while the controls were maintained in darkness. Hyphal morphology in the 6-well plates was recorded at 0, 12, 24, 36, 48 h. One hundred microliters of spore suspensions of each strain at these five time points was transferred to SGA plates and cultured for 36 h at 29 °C; the number of colonies formed was counted as a measure of conidia quality and viability. Blue light has dual effects on F. solani. The hyphal length of F. solani exposed to blue light was significantly shorter than that of the control (P < 0.01), indicating that fungal growth was inhibited. Meanwhile, instead of reducing the viability of spores, blue light significantly enhanced the conidia quality and viability after at least 24 h irradiation. Daylight-intensity blue light exposure will inhibit the hyphal growth of F. solani but promote conidiation, which would be more harmful to fungal keratitis. Eliminating the influence of blue light for these patients should be taken into account.
目的是研究日光强度蓝光对从真菌性角膜炎患者角膜分离出的镰刀菌的影响。将 5 株镰刀菌（1 株标准株和 4 株临床角膜分离株）的孢子悬浮液在 6 孔板中制备。蓝光组用峰值波长为 454nm 的发光二极管（LED）装置在 0.5mW/cm 下辐照 0 至 48 小时，而对照组则保持在黑暗中。在 0、12、24、36 和 48 小时记录 6 孔板中的菌丝形态。将每个菌株在这五个时间点的 100 微升孢子悬浮液转移到 SGA 平板上，在 29°C 下培养 36 小时；形成的菌落数被用作衡量分生孢子质量和活力的指标。蓝光对镰刀菌有双重作用。暴露在蓝光下的镰刀菌的菌丝长度明显短于对照组（P<0.01），表明真菌生长受到抑制。同时，蓝光不仅没有降低孢子的活力，反而在至少 24 小时照射后显著提高了分生孢子的质量和活力。日光强度的蓝光照射会抑制镰刀菌的菌丝生长，但会促进分生孢子的形成，这对真菌性角膜炎的危害更大。应该考虑为这些患者消除蓝光的影响。
