Cerón-Bustamante Minely, Tini Francesco, Beccari Giovanni, Benincasa Paolo, Covarelli Lorenzo
Department of Agricultural, Food and Environmental Sciences, University of Perugia, Borgo XX Giugno 74, 06121 Perugia, Italy.
J Fungi (Basel). 2023 Jun 14;9(6):670. doi: 10.3390/jof9060670.
The wheat pathogen can respond to light by modulating its gene expression. Because several virulence-related genes are differentially expressed in response to light, different wavelengths could have a crucial role in the -wheat interaction. To explore this opportunity, the aim of this study was to analyze the effect of blue (470 nm), red (627 nm), blue-red, and white light on the in vitro and in planta development of . The morphology (mycelium appearance, color) and phenotypic (mycelium growth) characteristics of a strain were evaluated after 14 days under the different light conditions in two independent experiments. In addition, bread wheat plants were artificially inoculated with and grown for 35 days under the same light treatments. The disease incidence, severity, and fungal DNA were analyzed in a single experiment. Statistical differences were determined by using an ANOVA. The obtained results showed that the different light wavelengths induced specific morphological changes in mycelial growth. The blue light significantly reduced colony growth, while the dark and red light favored fungal development ( < 0.05). The light quality also influenced host colonization, whereby the white and red light had stimulating and repressing effects, respectively ( < 0.05). This precursory study demonstrated the influence of light on colonization in bread wheat.
小麦病原体可通过调节其基因表达来响应光照。由于几个与毒力相关的基因在对光照的响应中差异表达，不同波长可能在小麦与该病原体的相互作用中起关键作用。为探索这一可能性，本研究的目的是分析蓝光（470纳米）、红光（627纳米）、红蓝混合光和白光对该病原体体外及在植物体内生长发育的影响。在两个独立实验中，于不同光照条件下培养14天后，评估了该病原体菌株的形态（菌丝外观、颜色）和表型（菌丝生长）特征。此外，用该病原体对面包小麦植株进行人工接种，并在相同光照处理下培养35天。在一项实验中分析了发病率、病情严重程度和真菌DNA。采用方差分析确定统计差异。所得结果表明，不同光波长诱导了菌丝生长的特定形态变化。蓝光显著降低了菌落生长，而黑暗和红光有利于真菌发育（P<0.05）。光质也影响宿主定殖，其中白光和红光分别具有刺激和抑制作用（P<0.05）。这项初步研究证明了光照对面包小麦中该病原体定殖的影响。
