Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA, USA.
Crump Institute for Molecular Imaging, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA, USA.
Methods Mol Biol. 2020;2126:177-190. doi: 10.1007/978-1-0716-0364-2_16.
Positron emission tomography (PET) reporter genes (PRGs), when coupled with positron-emitting PET reporter probes (PRPs), are useful for tracking specific cell populations in cell-based therapies, in transgenic animal models, and in xenograft tumor progression experiments. The activities of incorporated PRGs in targeted cells can be monitored noninvasively by PET imaging in preclinical in vivo studies and clinical applications following systemic administration of the appropriate PRG. Here we describe a method that minimizes both design and variability of vector delivery vehicles for alternative PRGs and biological variability of the in vivo target when comparing the efficacy, sensitivity, and specificity of alternative PRG/PRP combinations for in vivo PRG imaging. The principles described for comparing alternative PRG/PRP reporter gene systems can be applied to comparisons of alternative fluorescence, bioluminescence, single-photon emission computerized tomography (SPECT), and magnetic resonance imaging (MRI) reporter genes.
正电子发射断层扫描（PET）报告基因（PRG）与正电子发射 PET 报告探针（PRP）结合使用，可用于跟踪细胞疗法、转基因动物模型和异种移植肿瘤进展实验中的特定细胞群体。通过在临床前体内研究中进行全身给予适当的 PRG 后进行正电子发射断层扫描成像，可以非侵入性地监测靶向细胞中掺入的 PRG 的活性。在这里，我们描述了一种方法，该方法可最大程度地减少替代 PRG 的载体传递载体的设计和变异性，以及体内目标的生物学变异性，从而比较替代 PRG/PRP 组合在体内 PRG 成像中的功效、灵敏度和特异性。用于比较替代 PRG/PRP 报告基因系统的原理可应用于替代荧光、生物发光、单光子发射计算机断层扫描（SPECT）和磁共振成像（MRI）报告基因的比较。
