The Key Laboratory of Zoological Systematics and Application, College of Life Sciences, Hebei University, 071002, Baoding, PR China.
The Key Laboratory of Zoological Systematics and Application, College of Life Sciences, Hebei University, 071002, Baoding, PR China.
Fish Shellfish Immunol. 2020 Apr;99:631-640. doi: 10.1016/j.fsi.2020.02.047. Epub 2020 Feb 27.
Understanding how the brine shrimp responds to different geographical populations can provide novel insights on response to bacterial stimulation. In the paper, Artemia sinica from lower altitudes and Artemia parthenogenetica from higher altitudes of the Tibetan Plateau, were used to illustrate different defense against bacteria mechanisms that these organisms used to adapt to different geographical environments. Protein kinase CK2 is a serine/threonine kinase with a multitude of protein substrates. It is a ubiquitous enzyme essential for the viability of eukaryotic cells, where its functions in a variety of cellular processes, including cell cycle progression, apoptosis, transcription, and viral infection. The gene encodes the same mRNA sequence in A. sinica and A. parthenogenetica, named AsCK2α and ApCK2α, respectively. The open reading frame was obtained, a 1047-bp sequence encoding a predicted protein of 349 amino acids. To systematically analyze the expression of AsCK2α and ApCK2α during embryonic development and bacterial challenge, real-time PCR, Western blotting and immunohistochemistry were performed. The results showed that AsCK2α was higher than ApCK2α at different developmental stages. Under bacterial challenge, the expression of ApCK2α was significantly higher than AsCK2α. Protein localization analysis showed that AsCK2α and ApCK2α were mainly distributed in the head and chest. Our research revealed that CK2α plays a vital role in the growth, development and bacterial stimulation of the brine shrimp.
了解卤虫对不同地理种群的反应如何，可以为研究其对细菌刺激的反应提供新的见解。本文选用了来自青藏高原低海拔和高海拔地区的卤虫作为研究对象，以阐明这些生物在适应不同地理环境时所采用的不同防御细菌机制。蛋白激酶 CK2 是一种丝氨酸/苏氨酸激酶，具有多种蛋白质底物。它是真核细胞生存所必需的普遍存在的酶，其在多种细胞过程中发挥作用，包括细胞周期进展、细胞凋亡、转录和病毒感染。该基因在卤虫和副溶血性弧菌中编码相同的 mRNA 序列，分别命名为 AsCK2α 和 ApCK2α。获得了开放阅读框，这是一个编码 349 个氨基酸的预测蛋白的 1047bp 序列。为了系统分析 AsCK2α 和 ApCK2α 在胚胎发育和细菌挑战过程中的表达，进行了实时 PCR、Western blot 和免疫组织化学分析。结果表明，在不同的发育阶段，AsCK2α 的表达均高于 ApCK2α。在细菌挑战下，ApCK2α 的表达明显高于 AsCK2α。蛋白定位分析表明，AsCK2α 和 ApCK2α 主要分布在头部和胸部。我们的研究表明，CK2α 在卤虫的生长、发育和细菌刺激中发挥着重要作用。
