Key Laboratory of Groundwater Resources and Environment, Ministry of Education, Jilin University, Changchun 130021, Jilin, China.
Engineering Lab for Water Pollution Control and Resources Recovery, School of Environment, Northeast Normal University, Changchun 130117, PR China.
J Colloid Interface Sci. 2020 Jun 1;569:195-203. doi: 10.1016/j.jcis.2020.02.080. Epub 2020 Feb 20.
Green microalgae are promising and sustainable bioenergy and biomass feedstocks, that specifically utilize blue and red light for photosynthesis. Using carbon dots (CDs) to optimize photoluminescence is an attractive strategy for enhancing microalgal bioresource production; however, CD synthesis traditionally requires harsh conditions and laborious procedures. Little research has focused on developing CDs with tunable emission that precisely satisfy photosynthetic requirements. In this work, we directionally prepared non-toxic CDs using a simple method, which could adsorb light at spectra 500-600 nm and emit red light at 580-700 nm. CDs significantly promoted microalgae (Chlorella) growth by ~15%. Meanwhile, potential intracellular bioresources, pigments, carbohydrates, proteins, and lipids were generally enhanced. CDs, combined with an extracellular polymeric substance of microalgal cells, served as numerous micro-bulbs for Chlorella irradiation to sustainably provide optimized light. In this context, photosystems I and II were both stimulated. As such, we prepared CDs with tunable emission, which could significantly enhance microalgae and bioresource production.
绿色微藻是很有前途且可持续的生物能源和生物质原料，它们专门利用蓝光和红光进行光合作用。使用碳点 (CDs) 来优化光致发光是一种很有吸引力的策略，可以提高微藻生物资源的产量；然而，CDs 的合成传统上需要苛刻的条件和繁琐的步骤。很少有研究致力于开发具有可调发射特性的 CDs，以精确满足光合作用的要求。在这项工作中，我们使用一种简单的方法有方向性地制备了无毒的 CDs，这些 CDs 可以在 500-600nm 的光谱范围内吸收光，并在 580-700nm 的范围内发射红光。CDs 显著促进了微藻（小球藻）的生长，使其增加了约 15%。同时，潜在的细胞内生物资源，如色素、碳水化合物、蛋白质和脂质也普遍得到了提高。CDs 与微藻细胞的细胞外聚合物结合，作为小球藻照射的许多微灯泡，可持续地提供优化的光。在这种情况下，光系统 I 和 II 都受到了刺激。因此，我们制备了具有可调发射特性的 CDs，这可以显著提高微藻和生物资源的产量。
