Research Center for Membrane and Film Technology, Department of Chemical Science and Engineering, Kobe University, 1-1 Rokkodai, Nada, Kobe 657-8501, Japan.
Research Initiative for Supra-Materials (RISM), Interdisciplinary Cluster for Cutting Edge Research, Shinshu University, 4-17-1 Wakasato, Nagano 380-8553, Japan; Department of Materials Chemistry, Faculty of Engineering, Shinshu University, 4-17-1 Wakasato, Nagano 380-8553, Japan.
J Colloid Interface Sci. 2020 Jun 1;569:211-218. doi: 10.1016/j.jcis.2020.02.079. Epub 2020 Feb 20.
We investigated the formation behavior of supported lipid bilayers (SLBs) on polyelectrolyte multilayers (PEMs) prepared using the layer-by-layer method. The SLBs were formed using the liposome fusion method, which was driven by electrostatic interactions. We used three types of cationic polyelectrolytes to prepare PEMs: poly(diallyldimethylammonium chloride), which consists of a linear short polymer chain with quaternary ammonium cations, polyvinylamidin, which presents a linear, long polymer chain and polyethyleneimine which features a branched polymer chain. Poly(sodium 4-styrenesulfonate) was used as an anionic polyelectrolyte. First, we evaluated the effect of the molecular structure of the polyelectrolytes on the formation of SLBs. The formation of SLBs was evaluated using water permeability data, the lateral diffusivity of lipid molecules on the PEMs was determined using a fluorescence recovery after photo-bleaching assay and the amount of lipid molecules adsorbed on the PEMs. We revealed that both the molecular structure and charge density of the polyelectrolytes affected the formation of SLBs. Furthermore, we could form SLBs on high permeable PEMs by combining different cationic polyelectrolytes. These SLBs would be applicable for a platform to immobilize lipophilic biomolecules.
我们研究了通过层层自组装方法制备的聚电解质多层膜（PEM）上支撑脂质双层（SLB）的形成行为。通过静电相互作用驱动的脂质体融合方法形成 SLB。我们使用三种类型的阳离子聚电解质来制备 PEM：聚（二烯丙基二甲基氯化铵），由带季铵阳离子的线性短聚合物链组成；聚乙烯亚胺，具有支化聚合物链。聚（4-苯乙烯磺酸钠）用作阴离子聚电解质。首先，我们评估了聚电解质的分子结构对 SLB 形成的影响。通过水渗透率数据评估 SLB 的形成，通过光漂白后荧光恢复测定在 PEM 上脂质分子的侧向扩散率以及吸附在 PEM 上的脂质分子的量。我们揭示了聚电解质的分子结构和电荷密度都影响 SLB 的形成。此外，我们可以通过组合不同的阳离子聚电解质在高渗透性的 PEM 上形成 SLB。这些 SLB 将适用于固定疏水分子的平台。
