Institute of Oceanography, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
Institute of Oceanography, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
Environ Toxicol Pharmacol. 2020 May;76:103357. doi: 10.1016/j.etap.2020.103357. Epub 2020 Feb 22.
The present study was conducted to evaluate how air pre-exposure influences the responses in gills and digestive gland of Ruditapes philippinarum on subsequent Tetrabromobisphenol A (TBBPA) exposure. Firstly, clams were maintained in seawater or pre-exposed to air for 24 h, and then exposed to 0 or 100 μg/L TBBPA. Clam tissues were sampled after 1 day (T1) and 7 days (T7) of exposure to TBBPA. The results showed that in comparison with TBBPA exposure alone, air pre-exposure following TBBPA exposure reduced TBBPA accumulation in both tissues, up-regulated mRNA levels of CAT, Hsp70 and pi-GST in gills, and induced GST activity and P-glycoprotein (Pgp) mRNA level in digestive gland of clams at T1, whereas increased lipid peroxidation at T7. Overall, the findings indicate that 24-h exposure to air can activate a priming mechanism withstanding the subsequent TBBPA exposure, which is transient and may change after prolonged subsequent exposure.
本研究旨在评估空气预暴露如何影响菲律宾蛤仔（Ruditapes philippinarum）鳃和消化腺对随后四溴双酚 A（TBBPA）暴露的反应。首先，将蛤蚌在海水中或空气预暴露 24 小时，然后暴露于 0 或 100μg/L TBBPA。在暴露于 TBBPA 后第 1 天（T1）和第 7 天（T7），采集蛤蚌组织样本。结果表明，与单独暴露于 TBBPA 相比，空气预暴露后再暴露于 TBBPA 可减少两种组织中的 TBBPA 积累，在 T1 时上调鳃中 CAT、Hsp70 和 pi-GST 的 mRNA 水平，并诱导消化腺中的 GST 活性和 P-糖蛋白（Pgp）mRNA 水平，而在 T7 时增加脂质过氧化。总体而言，这些发现表明 24 小时的空气暴露可以激活一个耐受随后 TBBPA 暴露的启动机制，该机制是短暂的，在长时间的后续暴露后可能会发生变化。
