Research Center for Analytical Sciences, Chemistry Department, College of Sciences, Northeastern University, Shenyang, 110819, China.
Research Center for Analytical Sciences, Chemistry Department, College of Sciences, Northeastern University, Shenyang, 110819, China.
Talanta. 2020 May 15;212:120749. doi: 10.1016/j.talanta.2020.120749. Epub 2020 Jan 15.
Pb is one of the major environmental pollutants, which can be visually detected by surface plasmon resonance of nanoparticles. Paper based analytical device, as a newly developed microfluidic detection platform, is featured in cost-effective and suitable for on-site analysis. In this paper, a sensitive and portable detection method for Pb was proposed, in which Pb was electrokinetically stacked on the paper fluidic channel by geometric field amplification effect and visualized online by glutathione-modified silver nanoparticles. Colorimetric quantification of the visualized stacking band was conducted by smart phone camera. To avoid unfavorable influence from pH change on the surface plasmon resonance visualization, field amplification effect was introduced by geometric design of the paper fluidic channel. The enriched Pb was clearly visible on the paper substrate, and the stacking band intensity was about four orders of magnitude enhanced, comparing to the intensity without stacking. A linear response to Pb was observed in the range of 0.3-7.0 μM (R = 0.997) with a limit of detection of 86 nM and a limit of quantity of 0.28 μM. The established method was used in the detection of Pb from river and lake water samples, and the results were confirmed by atomic absorption spectroscopy method.
铅是主要的环境污染物之一，其可以通过纳米粒子的表面等离子体共振来进行直观检测。纸基分析器件作为一种新兴的微流控检测平台，具有成本效益高、适合现场分析等特点。本文提出了一种用于检测 Pb 的灵敏、便携的新方法，该方法利用几何场放大效应将 Pb 电动堆积在纸流体通道上，并用谷胱甘肽修饰的银纳米粒子进行在线可视化。通过智能手机摄像头对可视化堆积带进行比色定量分析。为避免 pH 值变化对表面等离子体共振可视化的不利影响，通过纸流体通道的几何设计引入了场放大效应。堆积后的 Pb 在纸基质上清晰可见，堆积带强度比未堆积时增强了约四个数量级。在 0.3-7.0 μM 的范围内对 Pb 表现出线性响应（R=0.997），检测限为 86 nM，检测限量为 0.28 μM。该方法已用于河水和湖水样品中 Pb 的检测，并通过原子吸收光谱法对结果进行了验证。
