大鼠脑内侧隔区/斜角带内源性乙酰胆碱的释放

The release of endogenous acetylcholine from the medial septum/diagonal band of rat brain.

作者信息

Metcalf R H, Boegman R J, Riopelle R J, Ludwin S K

机构信息

Department of Pharmacology, Queen's University, Kingston, Ont., Canada.

出版信息

Neurosci Lett. 1988 Oct 31;93(1):85-90. doi: 10.1016/0304-3940(88)90017-1.

DOI:10.1016/0304-3940(88)90017-1

PMID:3211372

Abstract

This study sought to establish whether cholinergic neurons in the medial septum/vertical limb of the diagonal band (ms/vdB) release endogenous acetylcholine (ACh) locally, and whether the release was modulated by presynaptic feedback mechanisms. Release of ACh from slices of the ms/vdB was assessed by gas chromatography-mass spectrometry (GC-MS). Potassium depolarization resulted in a 20- to 25-fold increase in ACh release above spontaneous levels. Omission of Ca2+ from the incubation medium decreased this release by 91%. In the presence of 4 microM atropine, potassium-induced ACh release was enhanced by 48%. These results indicate that ACh is released in the ms/vdB by a Ca2+-dependent and atropine-sensitive process.

摘要

本研究旨在确定内侧隔核/斜角带垂直支（ms/vdB）中的胆碱能神经元是否在局部释放内源性乙酰胆碱（ACh），以及这种释放是否受突触前反馈机制调节。通过气相色谱-质谱联用（GC-MS）评估ms/vdB切片中ACh的释放。钾离子去极化导致ACh释放量比自发水平增加20至25倍。孵育培养基中去除Ca2+可使这种释放减少91%。在存在4 microM阿托品的情况下，钾离子诱导的ACh释放增强了48%。这些结果表明，ACh在ms/vdB中通过Ca2+依赖且对阿托品敏感的过程释放。