Lee Chun-Nin, Chiu Li-Hsuan, Fang Chia-Lang, Yeh Shauh-Der, Zuo Chun S, Chen Shih-Ching, Kuo Li-Kuo, Wang Yun-Ming, Lai Wen-Fu Thomas
Department of Pulmonary and Critical Care Medicine, Taipei Medical University-Shuang Ho Hospital.
Department of Research and Department of Dentistry, Taipei Medical University / Shuang-Ho Hospital; McLean Imaging Center, McLean Hospital/Harvard Medical School.
J Vis Exp. 2020 Feb 16(156). doi: 10.3791/58227.
A molecular imaging probe comprising superparamagnetic iron oxide (SPIO) nanoparticles and Mycobacterium tuberculosis surface antibody (MtbsAb) was synthesized to enhance imaging sensitivity for extrapulmonary tuberculosis (ETB). An SPIO nanoprobe was synthesized and conjugated with MtbsAb. The purified SPIO-MtbsAb nanoprobe was characterized using TEM and NMR. To determine the targeting ability of the probe, SPIO-MtbsAb nanoprobes were incubated with Mtb for in vitro imaging assays and injected into Mtb-inoculated mice for in vivo investigation with magnetic resonance (MR). The contrast enhancement reduction on magnetic resonance imaging (MRI) of Mtb and THP1 cells showed proportional to the SPIO-MtbsAb nanoprobe concentration. After 30 min of intravenous SPIO-MtbsAb nanoprobe injection into Mtb-infected mice, the signal intensity of the granulomatous site was enhanced by 14-fold in the T2-weighted MR images compared with that in mice receiving PBS injection. The MtbsAb nanoprobes can be used as a novel modality for ETB detection.
合成了一种包含超顺磁性氧化铁（SPIO）纳米颗粒和结核分枝杆菌表面抗体（MtbsAb）的分子成像探针，以提高肺外结核（ETB）的成像灵敏度。合成了一种SPIO纳米探针并将其与MtbsAb偶联。使用透射电子显微镜（TEM）和核磁共振（NMR）对纯化的SPIO-MtbsAb纳米探针进行表征。为了确定该探针的靶向能力，将SPIO-MtbsAb纳米探针与结核分枝杆菌（Mtb）孵育用于体外成像分析，并注入接种了Mtb的小鼠体内，以通过磁共振（MR）进行体内研究。Mtb和THP1细胞的磁共振成像（MRI）上的对比增强降低与SPIO-MtbsAb纳米探针浓度成比例。向感染Mtb的小鼠静脉注射SPIO-MtbsAb纳米探针30分钟后，与接受磷酸盐缓冲盐水（PBS）注射的小鼠相比，T2加权MR图像中肉芽肿部位的信号强度增强了14倍。MtbsAb纳米探针可作为一种用于ETB检测的新型方法。
