Kalananthan Tharmini, Murashita Koji, Rønnestad Ivar, Ishigaki Mitsumasa, Takahashi Kota, Silva Marta S, Wakabayashi Yuki, Lai Floriana, Shimizu Munetaka, Nilsen Tom O, Pino Martinez Enrique, Gomes Ana S
Department of Biological Sciences, University of Bergen, Bergen, Norway.
Research Center for Aquaculture Systems, National Research Institute of Aquaculture, Japan Fisheries Research and Education Agency, Tamaki, Japan.
Front Physiol. 2020 Feb 11;11:61. doi: 10.3389/fphys.2020.00061. eCollection 2020.
The orexigenic agouti-related protein (AgRP) and the anorexigenic pro-opiomelanocortin (POMC) are crucial players in the control of feed intake in vertebrates, yet their role in teleosts has not been fully established. Triplicate groups of Atlantic salmon () post smolts were subjected to (1) fasting for 3 days (fast) and (2) normal feeding (fed), resulting in a significant ( < 0.05) upregulation of hypothalamic transcripts levels in the fast group. Moreover, the mRNA abundance of was significantly ( < 0.05) correlated with the stomach dry weight content. Corresponding inverse patterns were observed for , albeit not statistically significant. No significant differences were found for the other paralogues, and and between fed and fast groups. The significant correlation between stomach fullness and mRNA expression suggests a possible link between the stomach filling/distension and satiety signals. Our study indicates that hypothalamic acts as an orexigenic signal in Atlantic salmon.
促食欲的刺鼠相关蛋白（AgRP）和抑食欲的阿黑皮素原（POMC）是脊椎动物摄食控制中的关键因子，但其在硬骨鱼中的作用尚未完全明确。将三组重复的大西洋鲑（）洄游后幼鱼分别进行（1）禁食3天（禁食组）和（2）正常投喂（投喂组），结果禁食组下丘脑转录本水平显著上调（<0.05）。此外，的mRNA丰度与胃干重含量显著相关（<0.05）。对于，观察到了相应的相反模式，尽管无统计学意义。在投喂组和禁食组之间，其他旁系同源物、以及未发现显著差异。胃饱满度与mRNA表达之间的显著相关性表明胃充盈/扩张与饱腹感信号之间可能存在联系。我们的研究表明，下丘脑在大西洋鲑中作为一种促食欲信号发挥作用。
