Cheng Zhihua, Zhuo Shibin, Liu Xiaofeng, Che Gen, Wang Zhongyi, Gu Ran, Shen Junjun, Song Weiyuan, Zhou Zhaoyang, Han Deguo, Zhang Xiaolan
State Key Laboratories of Agrobiotechnology, Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, MOE Joint Laboratory for International Cooperation in Crop Molecular Breeding, China Agricultural University, Beijing, China.
Key Laboratory of Biology and Genetic Improvement of Horticultural Crops of Northeast Region, Ministry of Agriculture, College of Horticulture & Landscape Architecture, Northeast Agricultural University, Harbin, China.
Front Plant Sci. 2020 Feb 10;10:1781. doi: 10.3389/fpls.2019.01781. eCollection 2019.
Cucumber is an important vegetable crop bearing fleshy pepo fruit harvested immature. Fruits left unpicked in time during summer production, as well as unfavorable environmental conditions during post-harvest shelf, will cause cucumber fruits to turn yellow and ripen, and thus impair the market value. Identification of maturity-related genes is of great agricultural and economic importance for cucumber production. Here, we isolated and characterized a MADS-box gene, () in cucumber. Expression analysis indicated that was specifically enriched in reproductive organs including stamens and carpels. Ectopic expression of was unable to rescue the indehiscence silique phenotype of mutant plant in . Instead, overexpression of resulted in early flowering, precocious phenotypes, and capelloid organs in wild-type . Biochemical analysis indicated that CsSHP directly interacted with cucumber SEPALLATA (SEP) proteins. expression increased significantly during the yellowing stage of cucumber ripening, and was induced by exogenous application of abscisic acid (ABA). Therefore, CsSHP may participate in fruit maturation through the ABA pathway and floral organ specification interaction with CsSEPs to form protein complex in cucumber.
黄瓜是一种重要的蔬菜作物，其肉质瓠果在未成熟时采收。夏季生产时未及时采摘的果实，以及采后货架期不利的环境条件，都会导致黄瓜果实变黄成熟，从而损害其市场价值。鉴定与成熟相关的基因对黄瓜生产具有重要的农业和经济意义。在此，我们在黄瓜中分离并鉴定了一个MADS-box基因（）。表达分析表明，该基因在包括雄蕊和心皮在内的生殖器官中特异性富集。该基因的异位表达无法挽救拟南芥突变体植物的裂果荚表型。相反，在野生型拟南芥中过表达该基因会导致早花、早熟表型和萼状器官。生化分析表明，CsSHP与黄瓜SEPALLATA（SEP）蛋白直接相互作用。在黄瓜成熟变黄阶段，该基因的表达显著增加，并受外源脱落酸（ABA）诱导。因此，CsSHP可能通过ABA途径参与果实成熟，并通过与CsSEPs相互作用在黄瓜中形成蛋白质复合物来决定花器官的发育。
