Ouyang Hongjia, Yu Jiao, Chen Xiaolan, Wang Zhijun, Nie Qinghua
College of Animal Science & Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, China.
Guangdong Province Key Laboratory of Waterfowl Healthy Breeding, Guangzhou, China.
PeerJ. 2020 Feb 4;8:e8351. doi: 10.7717/peerj.8351. eCollection 2020.
Development of skeletal muscle is closely related to broiler production traits. The myocyte-specific enhancer binding factor (MEF) 2D gene () and its variant transcripts play important parts in myogenesis.
To identify the transcript variants of chicken gene and their function, this study cloned chicken gene and identified its transcript variants from different tissue samples. The expression levels of different transcripts of gene in different tissues and different periods were measured, and their effects on myoblast proliferation and differentiation were investigated. Variations in MEF2D were identified and association analysis with chicken production traits carried out.
Four novel transcript variants of were obtained, all of which contained highly conserved sequences, including MADS-Box and MEF2-Domain functional regions. Transcript was expressed specifically in muscle, and its expression was increased during embryonic muscle development. The could promote differentiation of chicken myoblasts and its expression was regulated by . The single nucleotide polymorphism g.36186C > T generated a TAG stop codon, caused MEF2D-V4 to terminate translation early, and was associated with several growth traits, especially on early body weight.
We cloned the muscle-specific transcript of and preliminarily revealed its role in embryonic muscle development.
骨骼肌的发育与肉鸡生产性状密切相关。肌细胞特异性增强子结合因子（MEF）2D基因及其可变转录本在肌生成过程中发挥重要作用。
为了鉴定鸡MEF2D基因的转录本变体及其功能，本研究克隆了鸡MEF2D基因，并从不同组织样本中鉴定其转录本变体。检测了MEF2D基因不同转录本在不同组织和不同时期的表达水平，并研究了它们对成肌细胞增殖和分化的影响。鉴定了MEF2D的变异，并进行了与鸡生产性状的关联分析。
获得了4个新的MEF2D转录本变体，它们均包含高度保守的序列，包括MADS-Box和MEF2结构域功能区。转录本4在肌肉中特异性表达，其表达在胚胎肌肉发育过程中增加。MEF2D-V4可促进鸡成肌细胞的分化，其表达受miR-133调控。单核苷酸多态性g.36186C>T产生了一个TAG终止密码子，导致MEF2D-V4提前终止翻译，并与多个生长性状相关，尤其是早期体重。
我们克隆了MEF2D的肌肉特异性转录本，并初步揭示了其在胚胎肌肉发育中的作用。
