Key Laboratory for Major Obstetric Diseases of Guangdong Province, Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes, Experimental Department of Institute of Gynecology and Obstetrics, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
J Cell Mol Med. 2020 Apr;24(7):4212-4222. doi: 10.1111/jcmm.15081. Epub 2020 Mar 2.
MSCs are kind of cultured cells that reside in different tissues as inducers or regulators of physiological and pathological processes. Here, we derived MSCs from amniotic fluid and compared their differentiation ability and immunosuppression effect on PHA-activated PBMC with those of MSCs isolated from umbilical cords. Amniotic fluid MSCs were isolated and cultured on commercial AFC medium and classic MSC medium, and the number and size of colonies were used to evaluate differences in primary and passaged culture. Rate of proliferation, population doubling time, cell morphology, cell surface markers and mRNA expression were measured in subcultured cells. Furthermore, a comparative study was performed with umbilical cord MSCs to assess the ability of differentiation and immunosuppressive effect of PHA-stimulated PBMCs. Amniotic fluid MSCs were isolated and expanded by three methods, and exhibited nearly all the characteristics of umbilical cord MSCs. Compared with umbilical cord MSCs, amniotic fluid MSCs had an enhanced osteogenic and chrondrogenic differentiation capability, and stronger immunosuppression effect of inhibition of PHA-activated PBMC division. Culture with commercial AFCs medium yielded the highest percentage of CD105 expression and showed some advantages in primary cell isolation, cell source-specific marker retention and cell proliferation. We demonstrated that amniotic fluid MSCs exhibited some advantages over umbilical cord MSCs, and different culture media caused cell proliferation, cell surface marker and cell morphology change, but were not associated with varying differentiation capability and immune effects.
间充质干细胞是一种存在于不同组织中的细胞，作为生理和病理过程的诱导剂或调节剂。在这里，我们从羊水分离出间充质干细胞，并将其与从脐带分离的间充质干细胞的分化能力和免疫抑制作用进行比较。将羊水间充质干细胞在商业 AFC 培养基和经典 MSC 培养基上分离和培养，并通过集落计数和大小评估原代和传代培养的差异。在传代培养的细胞中测量增殖率、倍增时间、细胞形态、细胞表面标志物和 mRNA 表达。此外，还与脐带间充质干细胞进行了比较研究，以评估 PHA 刺激的 PBMC 的分化能力和免疫抑制作用。通过三种方法分离和扩增羊水间充质干细胞，表现出几乎与脐带间充质干细胞相同的特性。与脐带间充质干细胞相比，羊水间充质干细胞具有增强的成骨和成软骨分化能力，以及更强的抑制 PHA 激活的 PBMC 分裂的免疫抑制作用。使用商业 AFC 培养基培养可获得最高的 CD105 表达率，并在原代细胞分离、细胞来源特异性标志物保留和细胞增殖方面具有一些优势。我们证明了羊水间充质干细胞与脐带间充质干细胞相比具有一些优势，不同的培养基导致细胞增殖、细胞表面标志物和细胞形态发生变化，但与不同的分化能力和免疫效应无关。
