Department of Histology and Cytophysiology, Medical University of Białystok, Białystok, Poland.
Department of Neurosurgery, Medical University of Bialystok, Białystok, Poland.
PLoS One. 2020 Mar 2;15(3):e0229462. doi: 10.1371/journal.pone.0229462. eCollection 2020.
Aging is a major risk factor for morbidity and mortality from cardiovascular causes in men. To better understand the cellular processes related to age-related cardiac complications, we undertook research aimed at comparative evaluation of genes expression and distribution of β-catenin, CacyBP/SIP, galectin-3 and LMP7 in the heart of healthy men in different age groups. The study was conducted on the hearts of 12 men (organ donors) without a history of cardiovascular disease, who were divided into two age groups: men under and men over 45 years of age. On paraffin sections, immunohistochemical reactions were performed to detect β-catenin, CacyBP/SIP, galectin-3 and immunoproteasome subunit LMP7. The expression of genes coding β-catenin, CacyBP/SIP, galectin-3 and LMP7 was also evaluated by real-time PCR method. In the heart of men over 45 years old, both gene expression and immunoreactivity of β-catenin, CacyBP/SIP, galectin-3 and LMP7 were stronger compared to younger individuals. The results of the presented studies suggest that β-catenin, CacyBP/SIP, galectin-3 and immunoproteasomes might be involved in the internal regulation of heart homeostasis during ageing.
衰老是男性心血管疾病发病率和死亡率的一个主要危险因素。为了更好地了解与年龄相关的心脏并发症相关的细胞过程，我们进行了研究，旨在比较评估不同年龄组健康男性心脏中β-连环蛋白、CacyBP/SIP、半乳糖凝集素-3 和 LMP7 的基因表达和分布。该研究在 12 名（器官捐献者）男性的心脏上进行，这些男性没有心血管疾病史，他们被分为两个年龄组：45 岁以下和 45 岁以上的男性。在石蜡切片上，进行免疫组织化学反应以检测β-连环蛋白、CacyBP/SIP、半乳糖凝集素-3 和免疫蛋白酶体亚单位 LMP7。还通过实时 PCR 方法评估编码β-连环蛋白、CacyBP/SIP、半乳糖凝集素-3 和 LMP7 的基因的表达。在 45 岁以上的男性心脏中，β-连环蛋白、CacyBP/SIP、半乳糖凝集素-3 和 LMP7 的基因表达和免疫反应性均强于年轻个体。本研究的结果表明，β-连环蛋白、CacyBP/SIP、半乳糖凝集素-3 和免疫蛋白酶体可能参与衰老过程中心脏内稳态的内部调节。
