Department of Food and Animal Biotechnology, Seoul National University, Seoul 08826, Korea.
Department of Agricultural Biotechnology, Seoul National University, Seoul 08826, Korea.
ACS Synth Biol. 2020 Mar 20;9(3):508-516. doi: 10.1021/acssynbio.9b00360. Epub 2020 Mar 11.
Bacteriophage endolysin is one of the potential alternatives of conventional antibiotics, but the intrinsic limitations of the bacterial expression system may undermine the comprehensive application of this therapeutic protein. To circumvent such limitations, we adopted a yeast surface display system as a novel expression platform for endolysin. Endolysin LysSA11 from staphylococcal phage SA11 was expressed and surface-displayed in to exhibit sufficient antimicrobial activity against . Without any protein isolation or purification procedures, we showed that direct treatment of LysSA11-displaying yeast cells could accomplish a 5-log reduction of viable within 3 h. Furthermore, the surface-displayed LysSA11 exhibited superior stability over the soluble form of purified LysSA11 during 14 days of storage in a refrigerated environment. We suggest that the yeast surface display system is an efficient, stable, and straightforward platform for the production and antibacterial applications of endolysin.
噬菌体溶菌酶是传统抗生素的潜在替代品之一，但细菌表达系统的固有局限性可能会限制这种治疗蛋白的全面应用。为了克服这些局限性，我们采用了酵母表面展示系统作为溶菌酶的新型表达平台。金黄色葡萄球菌噬菌体 SA11 的溶菌酶 LysSA11 在 中表达并进行表面展示，以显示对 的足够抗菌活性。无需任何蛋白质分离或纯化步骤，我们表明直接处理表达 LysSA11 的酵母细胞可以在 3 小时内完成 5 个对数减少的活菌。此外，在冷藏环境下储存 14 天期间，表面展示的 LysSA11 比纯化 LysSA11 的可溶性形式表现出更高的稳定性。我们认为，酵母表面展示系统是生产和应用溶菌酶的有效、稳定且简单的平台。
