Department of Analytical and Toxicological Chemistry, Faculty of Chemistry and Pharmacy, Odessa I.I. Mechnikov National University, Odessa, 65082, Ukraine.
Department of Analytical and Toxicological Chemistry, Faculty of Chemistry and Pharmacy, Odessa I.I. Mechnikov National University, Odessa, 65082, Ukraine.
Anal Biochem. 2020 May 15;597:113671. doi: 10.1016/j.ab.2020.113671. Epub 2020 Feb 29.
A novel fast room temperature cloud point extraction (RT-CPE) procedure for preconcentration and spectrophotometric determination of phosphate based on the heteropoly blue formation was developed. The proposed method includes the formation of yellow molybdoantymonatophosphoric heteropoly complex, its extraction into Triton X-100 micellar phase obtained at room temperature and reduction of heteropoly complex by ascorbic acid solution in ethanol and absorbance measurement of heteropoly blue at 790 nm. Under optimal conditions (1% (v/v) of Triton X-100 and 0.05 M of ammonium benzoate for initiating of RT-CPE; 0.13 M ethanolic solution of ascorbic acid for reduction of heteropoly complex and dilution of surfactant rich phase), the calibration graph is linear in the range of phosphate concentrations of 1.58-63 μg L. The proposed RT-CPE procedure has been successfully applied to preconcentration phosphates and its spectrophotometric determination in water samples.
一种新的快速室温浊点萃取（RT-CPE）方法，基于杂多蓝的形成，用于磷的预浓缩和分光光度法测定。该方法包括黄色钼锑磷杂多酸配合物的形成、室温下用 Triton X-100 胶束相萃取以及在乙醇中的抗坏血酸溶液还原杂多配合物，并在 790nm 处测量杂多蓝的吸光度。在最佳条件下（1%（v/v）的 Triton X-100 和 0.05M 的苯甲酸铵用于引发 RT-CPE；0.13M 的抗坏血酸乙醇溶液用于还原杂多配合物并稀释富含表面活性剂的相），在 1.58-63μg L 的范围内，校准曲线呈线性。该 RT-CPE 方法已成功应用于水样中磷的预浓缩及其分光光度法测定。
