Department of Cardiology, The third Hospital of Jilin University, (Jilin Provincial Molecular Biology Research Centre for Precision Medicine of Major Cardiovascular Disease), Changchun, Jilin 130033, China.
Department of Anaesthesiology, The third Hospital of Jilin University, Changchun, Jilin 130033, China.
Life Sci. 2020 May 15;249:117476. doi: 10.1016/j.lfs.2020.117476. Epub 2020 Feb 28.
Mangiferin is a well-known xanthone extracted from mango leaves (Mangifera indica Linn). Mangiferin is widely distributed in the bark, peel, leaf, seed, stalk, and kernel of mango and higher plants. The pharmacological properties of mangiferin, including its antioxidant, anticancer, antiaging, antiviral, hepatoprotective, analgesic, and immunomodulatory activities, have been described in several studies. We investigated the effect of mangiferin on isoproterenol-induced apoptosis. Experimental heart failure was induced in rats by intraperitoneal administration of isoproterenol (5 mg/kg) for 7 consecutive days. Rats were divided into five groups: group I (sham rats), group II (isoproterenol alone control), group III (isoproterenol + 25 mg/kg mangiferin), group IV (isoproterenol + 50 mg/kg mangiferin), and group V (isoproterenol + 0.0225 mg/kg digitalis as a positive control). Hemodynamic parameters and body weight, heart weight and liver weight, apoptosis induction, and caspase-3, Bax, and Bcl-2 protein levels were measured, and a histopathological analysis of cardiomyocytes was performed. In addition, apoptosis and protein expression of caspase-3, cleaved caspase-3, Bax, and Bcl-2 were measured in cardiac H9c2 cells. Mangiferin supplementation significantly increased heart rate and improved the maximum rate of decrease in left ventricular (LV) pressure, the maximum rate of increase in LV pressure, and LV systolic pressure. Mangiferin reduced inflammatory cell infiltration and the number of broken myocardial fibers, and decreased apoptosis in cardiomyocytes by reducing proteins levels of caspase-3 and Bax and increasing those of Bcl-2. Our findings suggest that mangiferin has a cardioprotective effect against isoproterenol-induced apoptosis in cardiomyocytes.
芒果叶提取物芒果苷是一种广为人知的黄烷酮。芒果苷广泛存在于芒果及高等植物的树皮、果皮、叶片、种子、茎和核中。多项研究已描述了芒果苷的药理学特性，包括其抗氧化、抗癌、抗老化、抗病毒、保肝、镇痛和免疫调节作用。我们研究了芒果苷对异丙肾上腺素诱导的细胞凋亡的影响。通过腹腔内注射异丙肾上腺素（5mg/kg）连续 7 天，在大鼠中诱导实验性心力衰竭。大鼠分为五组：I 组（假手术大鼠）、II 组（仅给予异丙肾上腺素对照）、III 组（给予异丙肾上腺素+25mg/kg 芒果苷）、IV 组（给予异丙肾上腺素+50mg/kg 芒果苷）和 V 组（给予异丙肾上腺素+0.0225mg/kg 地高辛作为阳性对照）。测量血流动力学参数和体重、心脏重量和肝脏重量、诱导细胞凋亡以及 caspase-3、Bax 和 Bcl-2 蛋白水平，并对心肌细胞进行组织病理学分析。此外，还测量了心脏 H9c2 细胞中 caspase-3、裂解 caspase-3、Bax 和 Bcl-2 的凋亡和蛋白表达。芒果苷补充显著增加心率，改善左心室（LV）压力下降最大速率、LV 压力上升最大速率和 LV 收缩压。芒果苷通过降低 caspase-3 和 Bax 蛋白水平和增加 Bcl-2 蛋白水平，减少炎症细胞浸润和破碎心肌纤维数量，降低心肌细胞凋亡。我们的研究结果表明，芒果苷对异丙肾上腺素诱导的心肌细胞凋亡具有心脏保护作用。
