Laboratory of Animal Cell and Molecular Biology, Innovation Lab, College of Bioscience and Biotechnology, Shenyang Agricultural University, 120 Dongling Road, Shenhe District, Shenyang, 110866, Liaoning, China.
College of Animal Science and Technology, Zhejiang A&F University, Lin'an, 311300, Zhejiang, China.
Environ Sci Pollut Res Int. 2020 May;27(14):16451-16459. doi: 10.1007/s11356-020-08183-8. Epub 2020 Mar 2.
Roundup is a widely used glyphosate-based herbicide worldwide. Roundup residues can be detected in the organs and urine of animals. However, its toxicity on mammalian preimplantation embryos has not been well investigated. Here, we show Roundup impairs the development and quality of bovine preimplantation embryos in a dose-dependent manner. Exposure to the agricultural recommended doses of Roundup caused in vitro developmental arrest and quick death of bovine embryos. Furthermore, even a very low concentration (0.9 ppm) of Roundup was harmful to bovine preimplantation development. In addition, Roundup increases intracellular calcium levels and induces oxidative stress and apoptosis in bovine embryos. Even if the embryos developed to morphologically normal blastocysts when cultured with low concentrations of Roundup, abnormal intracellular calcium and oxidative stress could be detected inside the embryos and led to an increased incidence of apoptosis in the blastocysts. These data suggest Roundup residues from the agricultural application are potentially dangerous to mammalian preimplantation embryos.
草甘膦是一种在全世界广泛使用的基于草甘膦的除草剂。草甘膦残留可以在动物的器官和尿液中检测到。然而，它对哺乳动物着床前胚胎的毒性尚未得到很好的研究。在这里，我们显示草甘膦以剂量依赖的方式损害牛着床前胚胎的发育和质量。暴露于农业推荐剂量的草甘膦导致体外胚胎发育停滞和牛胚胎快速死亡。此外，即使是非常低的浓度（0.9ppm）的草甘膦也对牛着床前胚胎发育有害。此外，草甘膦增加细胞内钙离子水平，并诱导牛胚胎氧化应激和细胞凋亡。即使胚胎在低浓度草甘膦的培养下发育成形态正常的囊胚，也可以检测到胚胎内异常的细胞内钙离子和氧化应激，导致囊胚凋亡的发生率增加。这些数据表明农业应用中的草甘膦残留对哺乳动物着床前胚胎具有潜在危险。
