Department of Physiology, University of Karachi, Karachi, Pakistan.
Department of Physiology, Bahria University of Medical and Dental College, Karachi Campus (BUMDC), Pakistan.
Taiwan J Obstet Gynecol. 2020 Mar;59(2):189-194. doi: 10.1016/j.tjog.2020.01.004.
To explore a disparity in anti-oxidants (SIRT1) and pro-oxidants (cortisol) levels as a plausible cause of unexplained infertility in females.
It was a cross sectional study in which 342 females with unexplained infertility were recruited from ACIMC outpatient clinic: 135 infertile cases and 207 fertile controls. Biochemical estimation of serum cortisol and SIRT1 was performed using Enzyme Linked Immuno Sorbent Assay. Statistical comparisons were performed using Student-t test and Mann-Whitney U test. Associations between circulating hormone levels and infertility were determined using Spearman's rank correlation. Associations were considered significant where value of p was less than 0.05.
The stress hormones profile of case and control demonstrated that the antioxidant SIRT1 was significantly lower in infertile females when compared with the fertile (p =< 0.001) while (the oxidant) Cortisol showed a contrast of results with higher values of in infertile females when compared with fertile counterparts (p =< 0.01). There was a strong negative association observed between SIRT1 and cortisol serum level (r = 0.244, p < 0.001).
Chronic stress causes oxidative stress that is depicted by a decrease in antioxidant levels in infertile females.
探讨抗氧化剂（SIRT1）和促氧化剂（皮质醇）水平的差异是否是女性不明原因不孕的一个可能原因。
这是一项横断面研究，共招募了 342 名来自 ACIMC 门诊的不明原因不孕女性：135 例不孕病例和 207 例正常生育对照。采用酶联免疫吸附试验（ELISA）法检测血清皮质醇和 SIRT1 的生化水平。采用学生 t 检验和曼-惠特尼 U 检验进行统计学比较。采用 Spearman 秩相关分析确定循环激素水平与不孕的相关性。当 p 值小于 0.05 时，认为关联具有统计学意义。
病例组和对照组的应激激素谱表明，与正常生育组相比，不孕女性的抗氧化剂 SIRT1 水平显著降低（p<0.001），而（氧化剂）皮质醇则表现出相反的结果，不孕女性的皮质醇水平显著高于正常生育对照组（p<0.01）。SIRT1 与皮质醇血清水平之间存在强烈的负相关关系（r=0.244，p<0.001）。
慢性应激导致氧化应激，表现为不孕女性抗氧化剂水平下降。
