Lour草药提取物对脂多糖诱导的RAW 264.7细胞一氧化氮产生的抑制活性。
The Inhibitory Activity of Lour Herbs Extract on Nitric Oxide Production toward RAW 264.7 Cells Induced by Lipopolysaccharide.
作者信息
Auliafendri Novycha, Rosidah Rosidah, Yuandani Yuandani, Suryani Sri, Satria Denny
机构信息
Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, 20155, Indonesia.
Department of Biochemistry, Faculty of Medicine, Universitas Sumatera Utara, Medan, 20155, Indonesia.
出版信息
Open Access Maced J Med Sci. 2019 Nov 14;7(22):3737-3740. doi: 10.3889/oamjms.2019.493. eCollection 2019 Nov 30.
AIM
The objective of this study was to evaluate the inhibitory activity of Lour on Nitric Oxide production toward RAW 264.7 cells.
METHODS
The extraction was obtained by maceration method using -hexane, ethyl acetate and ethanol solvents and then nitric oxide (NO) production was obtained using Griess reagent.
RESULTS
Extract of Lour herbs can reduce the NO production toward RAW 264.7 cells with induced by lipopolysaccharide has obtained nitric concentrations 12.5 and 25 μg/mL from -hexane extract (72.50 ± 4.51 and 10.42 ± 1.82), ethyl acetate extract: (88.33 ± 6.51 and 30.83 ± 6.86), ethanol extract: (75.00 ± 1.91 and 22.08 ± 2.53).
CONCLUSION
-hexane extract of Lour Herbs has a high potential to reduce the NO production in LPS-stimulated RAW 264.7 cells compared to ethyl acetate and ethanol extracts of Lour Herbs.
目的
本研究的目的是评估Lour对RAW 264.7细胞一氧化氮产生的抑制活性。
方法
采用浸渍法，使用正己烷、乙酸乙酯和乙醇溶剂进行提取，然后使用格里斯试剂测定一氧化氮（NO）的产生量。
结果
Lour草药提取物能够降低脂多糖诱导的RAW 264.7细胞的NO产生量，正己烷提取物在12.5和25μg/mL浓度下的NO浓度分别为（72.50±4.51和10.42±1.82），乙酸乙酯提取物为（88.33±6.51和30.83±6.86），乙醇提取物为（75.00±1.91和22.08±2.53）。
结论
与Lour草药的乙酸乙酯和乙醇提取物相比，Lour草药的正己烷提取物在降低脂多糖刺激的RAW 264.7细胞中NO产生量方面具有很高的潜力。
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