Department of Biotechnology, Periyar Maniammai Institute of Science & Technology, Vallam, Thanjavur, Tamil Nadu, 613403, India.
Arch Microbiol. 2020 Aug;202(6):1285-1293. doi: 10.1007/s00203-020-01838-7. Epub 2020 Mar 3.
Mycelial morphogenesis and the production of fungal secretory proteins are still largely unknown. A mutant strain of Aspergillus carbonarius UV-10046 produced abundant polygalacturonase (PG) along with partially saturated canthaxanthin (PSC) at low pH conditions. In the present study, the relationship between PG secretion and PSC biosynthesis was studied using carotenogenic inhibitors and SDS-PAGE electrophoresis. Also the correlation between morphogenesis and PG secretion was investigated by analysing through microscopic studies. From the results, it was observed that secretion of PG was positively influenced by the PSC biosynthesis. The results also showed that the mutant with hairy mycelial structure resulted in higher PG activity when compared to the wild type that lacks hyper branching. From the results, it was confirmed that a mutation might have occurred in the isoprenoid pathway that has helped mutant for survival at acidic conditions. Further, an alteration in the morphogenesis and hyper branching development caused over secretion of PG enzyme in the mutant.
菌丝体形态发生和真菌分泌蛋白的产生在很大程度上仍然未知。黑曲霉 UV-10046 的一个突变株在低 pH 条件下产生丰富的多聚半乳糖醛酸酶 (PG) 和部分饱和角黄素 (PSC)。在本研究中，使用类胡萝卜素生物合成抑制剂和 SDS-PAGE 电泳研究了 PG 分泌和 PSC 生物合成之间的关系。还通过微观研究分析了形态发生和 PG 分泌之间的相关性。结果表明，PG 的分泌受到 PSC 生物合成的正向影响。结果还表明，与缺乏超分枝的野生型相比，具有毛状菌丝体结构的突变体产生的 PG 活性更高。结果证实，异戊二烯途径的突变可能有助于突变体在酸性条件下生存。此外，形态发生和超分枝发育的改变导致突变体中 PG 酶的过度分泌。
