ADAMTS4在结直肠癌中上调，可能是结直肠癌有用的预后指标。
ADAMTS4 is upregulated in colorectal cancer and could be a useful prognostic indicator of colorectal cancer.
作者信息
Shang Xue-Qin, Liu Kui-Liang, Li Qian, Lao Yue-Qiong, Li Nan-Shan, Wu Jing
机构信息
. Department of Gastroenterology, Beijing Shijitan Hospital, Capital Medical University, Beijing, 100038, China.
. The Second People's Hospital of Yunnan Province, Oncology Department, Kunming, 650021, China.
出版信息
Rev Assoc Med Bras (1992). 2020 Feb 27;66(1):42-47. doi: 10.1590/1806-9282.66.1.42. eCollection 2020.
OBJECTIVE
ADAMTS4 is a member of the ADAMTS4 family, which secretes proteinases. The mechanism of tumor metastasis may be correlated to its promotion of angiogenesis. It was determined whether ADAMTS4 participates in colorectal cancer progression.
METHODS
The expression in clinical samples and CRC cell lines was investigated. Using immunohistochemistry (IHC), fluorescence in situ hybridization (FISH), and RT-PCR, the expression of ADAMTS4 was determined in colorectal tumors of different cancer stages and anatomic sites, and in three cell lines of different aggressiveness.
RESULTS
The overexpression of ADAMTS4 was observed in tissue samples by IHC, and this was mainly located in the cytoplasm, as detected by FISH. The qRT-PCR and western blot analyses further supported the clinical sample findings.
CONCLUSION
The present data support the notion that the overexpression of ADAMTS4 in CRC might be useful as a non-invasive biomarker for detecting CRC in patients.
目的
ADAMTS4是分泌蛋白酶的ADAMTS家族成员之一。肿瘤转移机制可能与其促进血管生成有关。本研究旨在确定ADAMTS4是否参与结直肠癌进展。
方法
研究ADAMTS4在临床样本和结直肠癌细胞系中的表达。采用免疫组织化学（IHC）、荧光原位杂交（FISH）和逆转录-聚合酶链反应（RT-PCR），检测不同癌症分期和解剖部位的结直肠肿瘤以及三种不同侵袭性的细胞系中ADAMTS4的表达。
结果
通过IHC在组织样本中观察到ADAMTS4的过表达，FISH检测显示其主要位于细胞质中。qRT-PCR和蛋白质印迹分析进一步支持了临床样本的研究结果。
结论
目前的数据支持以下观点，即结直肠癌中ADAMTS4的过表达可能作为检测患者结直肠癌的非侵入性生物标志物。
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