Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, School of Life Sciences, Southwest University, Chongqing, China.
The Key Laboratory of Mariculture, Ministry of Education, Fisheries College, Ocean University of China, Qingdao, Shandong, China.
Fish Physiol Biochem. 2020 Jun;46(3):1139-1154. doi: 10.1007/s10695-020-00780-1. Epub 2020 Mar 4.
NUCB1 and NUCB2, two novel nucleobindins, have attracted extensive attention for their role in the appetite regulation in mammals. However, little is known about the appetite regulation of NUCB1 and NUCB2 in fish species. Therefore, we investigated the role of these peptides in the regulation of feeding in Schizothorax davidi (S. davidi). In this study, full-length cDNA sequences of nucb1 and nucb2A of S. davidi were obtained for the first time. Additionally, the tissue distribution and the effects of different energy status on nucb1 and nucb2A mRNAs abundance were assessed, showing that nucb1 and nucb2A are widely distributed in 18 detected tissues, with the highest expression in the cerebellum. The abundances of nucb1 and nucb2A increased in the hypothalamus at 1 h and 3 h post-feeding. Furthermore, fasting and re-feeding experiments showed that the expressions of nucb1 and nucb2A in hypothalamus significantly decreased after fasting for 7 days, and returned to the control level after re-feeding for 3 or 5 days. In conclusion, the present study suggests that both NUCB1 and NUCB2A are involved in the short-term and long-term appetite regulation, as an anorexigenic factor, in S. davidi. These results can provide a basis for further investigation into the appetite regulatory role of NUCB family in teleost.
NUCB1 和 NUCB2 是两种新型核结合蛋白，因其在哺乳动物食欲调节中的作用而受到广泛关注。然而，关于鱼类中 NUCB1 和 NUCB2 的食欲调节作用知之甚少。因此，我们研究了这些肽在齐口裂腹鱼（S. davidi）摄食调节中的作用。本研究首次获得了 S. davidi 的 nucb1 和 nucb2A 的全长 cDNA 序列。此外，还评估了 nucb1 和 nucb2A 在不同能量状态下的组织分布和丰度变化，结果表明 nucb1 和 nucb2A 广泛分布于 18 种检测组织中，在小脑中的表达最高。摄食后 1 小时和 3 小时，下丘脑 nucb1 和 nucb2A 的丰度增加。此外，禁食和再摄食实验表明，禁食 7 天后，下丘脑 nucb1 和 nucb2A 的表达显著降低，再摄食 3 或 5 天后恢复到对照水平。综上所述，本研究表明，在齐口裂腹鱼中，NUCB1 和 NUCB2A 均参与短期和长期的食欲调节，作为一种厌食因子。这些结果可为进一步研究鱼类中 NUCB 家族在硬骨鱼类中的食欲调节作用提供依据。
