Sibirtsev V S, Garabadgiu A V, Shvets V I
All-Russia Research Institute for Food Additives- Branch of V.M. Gorbatov Federal Research Center for Food Systems of RAS, 191014, St. Petersburg, Russia.
St. Petersburg State Institute of Technology, 190013, St. Petersburg, Russia.
Dokl Biol Sci. 2019 Nov;489(1):196-199. doi: 10.1134/S0012496619060103. Epub 2020 Mar 4.
A biotesting procedure that makes it possible to record the changes in the intensities of elastic light scattering, light absorption, and intrinsic photofluorescence of the protein component, as well as the determination of the concentration and structuring coefficients of the genomic component, of the samples with viable unicellular test organisms incubated in a liquid nutrient medium in the presence and absence of various external chemical factors, is described. The results of the analysis of the antibiotic activity of various metal cations with the use of this technique are presented. This technique is much more rapid, objective, and comprehensive for the assessment of the effect on the reproduction rate, metabolic activity, and genome structure of the test organisms from the samples of various products, wastes, etc. than standard visual microbiotesting methods.
描述了一种生物测试程序，该程序能够记录在液体营养培养基中培养的、存在和不存在各种外部化学因素时的活单细胞测试生物体样品中蛋白质成分的弹性光散射强度、光吸收和固有光荧光的变化，以及基因组成分的浓度和结构系数的测定。给出了使用该技术分析各种金属阳离子抗生素活性的结果。与标准的视觉微生物测试方法相比，该技术在评估各种产品、废物等样品对测试生物体的繁殖率、代谢活性和基因组结构的影响方面要快得多、客观得多且全面得多。
