Bolsunovsky A Ya, Dementyev D V, Frolova T S, Trofimova E A, Iniatkina E M, Vasilyev S A, Sinitsyna O I
Institute of Biophysics, Krasnoyarsk Science Center, Siberian Branch, Russian Academy of Sciences, 660036, Krasnoyarsk, Russia.
Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, 630090, Novosibirsk, Russia.
Dokl Biochem Biophys. 2019 Nov;489(1):362-366. doi: 10.1134/S1607672919060024. Epub 2020 Mar 4.
The effect of γ-radiation on the level of nuclear DNA damage in onion seedlings (Allium-test) was studied using the comet assay. DNA breaks were first found in cells of onion seedlings exposed to low-dose radiation (≤ 0.1 Gy). Dose dependence of DNA damage parameters showed nonlinear behavior: a linear section in the low-dose region (below 0.1 Gy) and a dose-independent plateau in the dose range between 1 and 5 Gy. Thus, the comet assay can be used to estimate the biological effects of low-dose γ-radiation on Allium cepa seedlings.
采用彗星试验研究了γ辐射对洋葱幼苗（葱属试验）核DNA损伤水平的影响。在暴露于低剂量辐射（≤0.1 Gy）的洋葱幼苗细胞中首次发现DNA断裂。DNA损伤参数的剂量依赖性表现为非线性行为：低剂量区域（低于0.1 Gy）呈线性部分，在1至5 Gy剂量范围内呈剂量非依赖性平台期。因此，彗星试验可用于评估低剂量γ辐射对洋葱幼苗的生物学效应。
