Chen Gui Lin, Xu Shan, Wu Zi Juan, Wu Yi
Department of Pharmacy,Changzhou Second People's Hospital,Changzhou,Jiangsu 213000,China.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2020 Feb 28;42(1):30-36. doi: 10.3881/j.issn.1000-503X.11165.
To investigate the protective effect of salvianolic acid B(SAB)on the intestinal tract of rats after intestinal ischemia-reperfusion injury(IIRI). Forty-eight healthy male SD rats were equally randomized into IIRI group,SAB+IIRI group,sham control group,and SAB+sham control group. The malonyldialdehyde(MDA)level and superoxide dismutase(SOD)activity in the ileum were measured in each group according to the kit instructions,the transcription levels of inflammatory factors in the ileum of rats were detected by real-time fluorescence quantitative RT-PCR,the secretion level of inflammatory factors was detected by ELISA,and the effects of intestinal ischemia-reperfusion on intestinal permeability and histological lesions were measured by histopathology. The MDA level in IIRI group was significantly higher than those in negative control group(=0.005)and SAB+IIRI group(=0.012). SOD activity of IIRI group was significantly lower than those of negative control group(=0.006)and SAB+IIRI group(=0.017). The optical densities of tumor necrosis factor-α(TNF-α)(=0.003,=0.009),interleukin(IL)-1β(=0.026,=0.005),IL-6(=0.015,=0.003),and nuclear factor kappa-B(NF-κB)(=0.007,=0.015)in IIRI group were significantly higher than those in sham control group and SAB+IIRI group. The TNF-α(=0.002,=0.006),IL-1β(=0.002,=0.006),IL-6(=0.008,=0.002),and NF-κB(=0.026,=0.005)levels in IIRI group were significantly higher than those in sham control group and SAB+IIRI group. The inulin level in IIRI group was significantly lower than that in negative control group(=0.015)and significantly higher than that in SAB+IIRI group(=0.011). The dextran level in IIRI group was significantly lower than those in sham control group(=0.011)and SAB+IIRI group(=0.012). The dextran gel level in IIRI group was significantly higher than those in sham control group(=0.031)and SAB+IIRI group(=0.020). SAB pretreatment remarkably improved the edema,necrosis,and villus stripping of the intestinal mucosa in the ileum of rats. The Chiu score was significantly higher in SAB+sham control group than in sham control group(=0.001)and was significantly lower in SAB+IIRI group than in IIRI group(=0.001). SAB pretreatment can alleviate IIRI in rat models,and this protective effect may be achieved by alleviating oxidative stress and inflammation in the intestinal tract.
探讨丹酚酸B（SAB）对大鼠肠缺血再灌注损伤（IIRI）后肠道的保护作用。将48只健康雄性SD大鼠随机分为IIRI组、SAB + IIRI组、假手术对照组和SAB + 假手术对照组。按照试剂盒说明书测定各组大鼠回肠中丙二醛（MDA）水平和超氧化物歧化酶（SOD）活性，采用实时荧光定量RT-PCR检测大鼠回肠中炎症因子的转录水平，通过ELISA检测炎症因子的分泌水平，用组织病理学方法检测肠缺血再灌注对肠道通透性和组织学损伤的影响。IIRI组MDA水平显著高于阴性对照组（P = 0.005）和SAB + IIRI组（P = 0.012）。IIRI组SOD活性显著低于阴性对照组（P = 0.006）和SAB + IIRI组（P = 0.017）。IIRI组肿瘤坏死因子-α（TNF-α）（P = 0.003，P = 0.009）、白细胞介素（IL）-1β（P = 0.026，P = 0.005）、IL-6（P = 0.015，P = 0.003）和核因子κB（NF-κB）（P = 0.007，P = 0.015）的光密度显著高于假手术对照组和SAB + IIRI组。IIRI组TNF-α（P = 0.002，P = 0.006）、IL-1β（P = 0.002，P = 0.006）、IL-6（P = 答案0.008，P = 0.002）和NF-κB（P = 0.026，P = 0.005）水平显著高于假手术对照组和SAB + IIRI组。IIRI组菊粉水平显著低于阴性对照组（P = 0.015）且显著高于SAB + IIRI组（P = 0.011）。IIRI组右旋糖酐水平显著低于假手术对照组（P = 0.011）和SAB + IIRI组（P = 0.012）。IIRI组右旋糖酐凝胶水平显著高于假手术对照组（P = 0.031）和SAB + IIRI组（P = 0.020）。SAB预处理显著改善了大鼠回肠肠黏膜的水肿、坏死和绒毛脱落。SAB + 假手术对照组的Chiu评分显著高于假手术对照组（P = 0.001），且SAB + IIRI组显著低于IIRI组（P = 0.001）。SAB预处理可减轻大鼠模型中的IIRI，这种保护作用可能是通过减轻肠道氧化应激和炎症来实现的。
