Department of Education and Medical Research, National Yang-Ming University Hospital, Yilan, Taiwan, R.O.C.
Institute of Oral Biology, School of Dentistry, National Yang-Ming University, Taipei, Taiwan, R.O.C.
Anticancer Res. 2020 Mar;40(3):1427-1436. doi: 10.21873/anticanres.14084.
BACKGROUND/AIM: Cervical cancer is one of the most common cancers worldwide and a major cause of cancer-related mortality among women. Previous studies have reported that microRNA-miR-187*, which is one of the non-coding parts of the genome producing small conserved ribonucleic acids, is associated with various cancers. In this study, we explored the function of miR-187* in cervical cancer cells.
miR-187* mimic, WWOX reporter constructs, siRNA and overexpression constructs were transfected into SiHa cells to investigate the function and regulatory mechanisms of miR-187*.
Exogenous miR-187* was found to increase the oncogenic phenotypes of SiHa cells. The tumor suppressor gene WWOX is a novel target of miR-187* in SiHa cells. WWOX siRNA suppressed endogenous WWOX expression and increased the oncogenic phenotypes of SiHa cells. Exogenous WWOX expression was able to suppress the oncogenic phenotypes of SiHa cells and rescue cells from miR-187*-induced migration.
miR-187* seems to enhance SiHa cervical cancer cell oncogenicity via suppression of the WWOX pathway.
背景/目的：宫颈癌是全球最常见的癌症之一，也是导致女性癌症相关死亡的主要原因之一。先前的研究表明，微小 RNA- miR-187*，作为基因组产生小的保守核糖核酸的非编码部分之一，与各种癌症有关。在本研究中，我们探讨了 miR-187*在宫颈癌中的功能。
将 miR-187模拟物、WWOX 报告基因构建体、siRNA 和过表达构建体转染到 SiHa 细胞中，以研究 miR-187的功能和调节机制。
外源性 miR-187被发现增加了 SiHa 细胞的致癌表型。肿瘤抑制基因 WWOX 是 SiHa 细胞中 miR-187的一个新的靶基因。WWOX siRNA 抑制内源性 WWOX 表达，并增加 SiHa 细胞的致癌表型。外源性 WWOX 表达能够抑制 SiHa 宫颈癌细胞的致癌表型，并挽救细胞免受 miR-187*诱导的迁移。
miR-187*似乎通过抑制 WWOX 途径增强 SiHa 宫颈癌细胞的致癌性。
