Disruption of Intracellular Calcium Homeostasis as a Therapeutic Target Against .

There is no effective cure for Chagas disease, which is caused by infection with the arthropod-borne parasite, . In the search for new drugs to treat Chagas disease, potential therapeutic targets have been identified by exploiting the differences between the mechanisms involved in intracellular Ca homeostasis, both in humans and in trypanosomatids. In the trypanosomatid, intracellular Ca regulation requires the concerted action of three intracellular organelles, the endoplasmic reticulum, the single unique mitochondrion, and the acidocalcisomes. The single unique mitochondrion and the acidocalcisomes also play central roles in parasite bioenergetics. At the parasite plasma membrane, a Ca-ATPase (PMCA) with significant differences from its human counterpart is responsible for Ca extrusion; a distinctive sphingosine-activated Ca channel controls Ca entrance to the parasite interior. Several potential anti-trypansosomatid drugs have been demonstrated to modulate one or more of these mechanisms for Ca regulation. The antiarrhythmic agent amiodarone and its derivatives have been shown to exert trypanocidal effects through the disruption of parasite Ca homeostasis. Similarly, the amiodarone-derivative dronedarone disrupts Ca homeostasis in epimastigotes, collapsing the mitochondrial membrane potential (ΔΨ), and inducing a large increase in the intracellular Ca concentration ([Ca]) from this organelle and from the acidocalcisomes in the parasite cytoplasm. The same general mechanism has been demonstrated for SQ109, a new anti-tuberculosis drug with potent trypanocidal effect. Miltefosine similarly induces a large increase in the [Ca] acting on the sphingosine-activated Ca channel, the mitochondrion and acidocalcisomes. These examples, in conjunction with other evidence we review herein, strongly support targeting Ca homeostasis as a strategy against Chagas disease.