Lee Hoyoon, Park Chanhee, Na Wonhwi, Park Kyong Hwa, Shin Sehyun
1School of Mechanical Engineering, Korea University, Seoul, 02841 Republic of Korea.
2Nano-Biofluigonstic Research Center, Korea University, Seoul, 02841 Republic of Korea.
NPJ Precis Oncol. 2020 Feb 24;4:3. doi: 10.1038/s41698-019-0107-0. eCollection 2020.
Cell-free DNA (cfDNA) has been implicated as an important biomarker in cancer management. Thus, efficient techniques for cfDNA extraction are necessary for precision medicine. We developed a centrifugation-free cfDNA extraction microfluidic chip capable of extracting cfDNA from plasma samples through microfluidic circuits within 15 min under vacuum pressure using an immiscible solvent. The microfluidic chip had excellent performance that was comparable to the most widely used commercial product (QIAamp kit) in terms of extraction efficiency, purity, and quality of DNA samples. The microfluidic chip was validated for the continuous monitoring of type breast cancer and was able to successfully detect a point mutation in phosphatidylinositol-4,5-bisphosphate 3-kinase () during severe liver metastasis. The chip effectively eliminates the repetitive centrifugation processes and dramatically shortened the sample preparation time. The proposed platform could facilitate the development of a sample-to-answer system for use in liquid biopsy of cancers.
游离DNA（cfDNA）已被认为是癌症管理中的一种重要生物标志物。因此，高效的cfDNA提取技术对于精准医学来说是必要的。我们开发了一种无离心cfDNA提取微流控芯片，该芯片能够在真空压力下，使用不混溶溶剂，通过微流控电路在15分钟内从血浆样本中提取cfDNA。该微流控芯片具有优异的性能，在DNA样本的提取效率、纯度和质量方面，可与使用最广泛的商业产品（QIAamp试剂盒）相媲美。该微流控芯片已通过对乳腺癌的连续监测得到验证，并且能够在严重肝转移期间成功检测到磷脂酰肌醇-4,5-二磷酸3-激酶（）中的一个点突变。该芯片有效消除了重复离心过程，并显著缩短了样品制备时间。所提出的平台可促进用于癌症液体活检的样本到答案系统的开发。
