Department of Analytical Chemistry, Nutrition and Food Sciences, Institute of Research on Chemical and Biological Analysis (IAQBUS), Universidade de Santiago de Compostela, 15782, Santiago de Compostela, Spain.
Department of Analytical Chemistry, Nutrition and Food Sciences, Institute of Research on Chemical and Biological Analysis (IAQBUS), Universidade de Santiago de Compostela, 15782, Santiago de Compostela, Spain.
Chemosphere. 2020 Jul;251:126346. doi: 10.1016/j.chemosphere.2020.126346. Epub 2020 Feb 25.
The comprehensive identification of organic species existing in indoor environments is a key issue to understand their impact in human health. This study proposes the analysis of condensed water samples, collected with portable dehumidifiers, to characterize semi-volatile compounds in the gas phase of confined areas. Water samples are concentrated by solid-phase extraction (SPE). The obtained extracts are analysed by gas chromatography (GC) time-of-flight mass spectrometry (TOF-MS), following a non-target screening data mining approach. In first term, spectra of deconvoluted compounds are compared with those in NIST low resolution library; thereafter, tentative identifications are verified using an in-house database of accurate electron ionization (EI) MS spectra. Chromatographic (retention index) and spectral data are combined for unambiguous species identification. The potential of condensed water samples to reflect changes in the composition of indoor atmospheres, the match between data obtained using different dehumidifiers, and the relative concentration efficiency of condensed water compared to that attained by active sampling of moderate air volumes are discussed. A total of 141 semi-volatile compounds were identified (98 confirmed against authentic standards) in a set of 21 samples obtained from different homes and working places. This list contains more than 40 fragrances (including several potential allergens), solvents and intermediates in the production of polymeric materials, plasticizers and flame retardants.
综合鉴定室内环境中存在的有机物质是了解其对人类健康影响的关键问题。本研究提出了分析冷凝水样本的方法，这些样本是通过便携式除湿器收集的，用于表征密闭区域气相中的半挥发性化合物。水样通过固相萃取（SPE）进行浓缩。获得的提取物通过气相色谱（GC）飞行时间质谱（TOF-MS）进行分析，采用非靶向筛选数据挖掘方法。首先，将解卷积化合物的光谱与 NIST 低分辨率库中的光谱进行比较；然后，使用准确电子电离（EI）MS 光谱的内部数据库验证暂定鉴定。将色谱（保留指数）和光谱数据相结合，以进行明确的物种鉴定。讨论了冷凝水样品反映室内大气成分变化的潜力、不同除湿器获得的数据之间的匹配程度，以及与中体积空气主动采样相比，冷凝水的相对浓缩效率。从不同家庭和工作场所获得的 21 个样本中，共鉴定出 141 种半挥发性化合物（98 种通过标准品确认）。该列表包含 40 多种香料（包括几种潜在过敏原）、用于生产聚合物材料的溶剂和中间体、增塑剂和阻燃剂。
