Webster J J, Walker B G, Ford S R, Leach F R
Department of Biochemistry, Oklahoma State University, Stillwater.
J Biolumin Chemilumin. 1988 Jul-Sep;2(3):129-33. doi: 10.1002/bio.1170020304.
A bioluminescence procedure for measurement of microbial ATP allows a rapid determination of the effectiveness of autoclave sterilization. This determination is achieved faster than detection of acid production in a biological indicator via a pH indicator. Bacterial outgrowth from spores on test strips of the biological indicator was detected by measurement of ATP using the firefly luciferase reaction. A measureable increase in ATP was found after 5 hours of incubation of a biological indicator that had been treated under sterilizing conditions that produced 75% sterility of the biological indicator as measured by acid production. This is a marked improvement over the 24-48 hours of incubation currently required.
一种用于测量微生物ATP的生物发光程序能够快速测定高压灭菌的有效性。这种测定比通过pH指示剂检测生物指示剂中的产酸情况要快。使用萤火虫荧光素酶反应通过测量ATP来检测生物指示剂测试条上孢子的细菌生长。在对生物指示剂进行灭菌条件处理后孵育5小时，通过产酸测量该生物指示剂的无菌率为75%，此时发现ATP有可测量的增加。这比目前所需的24 - 48小时孵育有了显著改进。
