Burch W M, Lopez-Claros M, Uskokovic M R, Drezner M K
Department of Medicine, Duke University Medical Center, Durham, NC.
J Bone Miner Res. 1988 Feb;3(1):87-91. doi: 10.1002/jbmr.5650030113.
We addressed the question of whether 1,25-dihydroxyvitamin D3 (1,25-(OH)2D) could directly stimulate cartilage growth in vitro. Pelvic leaflets from chick embryos and scapular growth plates from fetal pigs were organ cultured in serum-free medium in the presence and absence of 1,25-(OH)2D. After 3 days of incubation, 1,25-(OH)2D had increased the pelvic cartilage wet weight 42% and the dry weight 32% above the weight of cartilages incubated in medium alone. 1,25-(OH)2D (10(-9) M-10(-12) M) caused a dose-dependent increase in weight, with maximal increases at 10(-9) M. Furthermore, two deuterized derivatives of 1,25-(OH)2D, 26,27-D6-1,25-(OH)2D3 and 24,26,27-D8-1,25-(OH)2D3, stimulated pelvic cartilage growth in vitro. 26,27-D6-1,25-(OH)2D stimulated increases in growth plate weight above growth plates incubated in medium alone. 26,27-D6-1,25-(OH)2D3 appeared to be potent at lower concentrations than 1,25-(OH)2D on growth plate cartilage. Thus, 1,25-(OH)2D stimulated in vitro growth in two growing cartilage models, the avian pelvic cartilage and the mammalian scapular growth plate cartilage.
我们研究了1,25 - 二羟基维生素D3（1,25-(OH)2D）是否能在体外直接刺激软骨生长的问题。将鸡胚的骨盆小叶和胎猪的肩胛生长板在有无1,25-(OH)2D的无血清培养基中进行器官培养。孵育3天后，与仅在培养基中孵育的软骨相比，1,25-(OH)2D使骨盆软骨湿重增加了42%，干重增加了32%。1,25-(OH)2D（10(-9)M - 10(-12)M）导致重量呈剂量依赖性增加，在10(-9)M时增加最大。此外，1,25-(OH)2D的两种氘代衍生物，26,27 - D6 - 1,25-(OH)2D3和24,26,27 - D8 - 1,25-(OH)2D3，在体外刺激了骨盆软骨生长。26,27 - D6 - 1,25-(OH)2D刺激生长板重量增加，超过仅在培养基中孵育的生长板。26,27 - D6 - 1,25-(OH)2D3在生长板软骨上似乎在比1,25-(OH)2D更低的浓度下就具有效力。因此，1,25-(OH)2D在两种生长软骨模型，即禽类骨盆软骨和哺乳动物肩胛生长板软骨中刺激了体外生长。
