School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China.
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China.
J Asian Nat Prod Res. 2021 Apr;23(4):353-362. doi: 10.1080/10286020.2020.1730331. Epub 2020 Mar 6.
Steroidal saponins named polyphyllin are the major active components of . Cycloartenol synthase (CAS) is a key enzyme that catalyzes the formation of the sterol scaffold. In this study, we cloned a putative gene from . Heterologous expression in yeast indicated that PpCAS can convert 2,3-oxidosqualene into cycloartenol. qRT-PCR analysis showed that the expression of was highest in leaves and lowest in roots. To our best knowledge, this is the first report of the functional characterization of cycloartenol synthase from , which lays the foundation for further analysis of the biosynthesis pathway of polyphyllins.[Formula: see text].
薯蓣皂苷元命名的甾体皂苷是主要活性成分的 。环阿屯醇合酶 (CAS) 是一种关键的酶，催化固醇支架的形成。在这项研究中，我们从 克隆了一个假定的 基因。在酵母中的异源表达表明，PpCAS 可以将 2,3-氧化角鲨烯转化为环阿屯醇。qRT-PCR 分析表明， 的表达在叶片中最高，在根中最低。据我们所知，这是首次报道来自 的环阿屯醇合酶的功能特征，为进一步分析薯蓣皂苷元的生物合成途径奠定了基础。[Formula: see text].
