College of Chemistry, Chemical Engineering and Environmental Engineering, Liaoning Shihua University, Fushun, 113001, China.
College of Life and Health Sciences, Northeastern University, Shenyang, 110000, China.
Anal Chim Acta. 2020 Apr 8;1105:155-161. doi: 10.1016/j.aca.2020.01.033. Epub 2020 Jan 17.
We have developed a simple and convenient route to prepare fluorescent carbon dots with dual emission peaks respectively at 470 and 570 nm. The prepared dual-emission carbon dots can be used for ratiometric detection of Fe3+ ions in the range from 0 to 50 μmol·L-1 with 0.8 μmol·L-1 detection limit based on the fluorescence quenching at 570 nm. The quenched fluorescence induced by Fe3+ ions could be recovered by pyrophosphate. We further used the carbon dots-Fe3+ ions-pyrophosphate mixed system for ratiometric detection of acid phosphatase in the range from 0.08 to 6.75 μg·mL-1 with 0.01 μg·mL-1 detection limit.
我们开发了一种简单便捷的方法来制备具有分别位于 470nm 和 570nm 的双发射峰的荧光碳点。所制备的双发射碳点可用于在 0 至 50μmol·L-1 范围内进行 Fe3+离子的比率检测，其检测限为 0.8μmol·L-1，基于在 570nm 处的荧光猝灭。Fe3+离子引起的猝灭荧光可以通过焦磷酸根来恢复。我们进一步使用碳点-Fe3+离子-焦磷酸混合体系在 0.08 至 6.75μg·mL-1 范围内进行酸性磷酸酶的比率检测，其检测限为 0.01μg·mL-1。
