Department of Food Science and Technology, University of Kashmir, Srinagar, 190006, India.
Department of Food Technology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, Songkhla, 90112, Thailand.
Sci Rep. 2020 Mar 5;10(1):4097. doi: 10.1038/s41598-020-60770-4.
In this study resistant starch (RS) was extracted from five Himalayan rice cultivars and Indian Horse chestnut (HCN) using porcine pancreatin enzyme following which it was subsequently characterized for its physicochemical, structural and functional properties. In vitro digestibility test showed that RS content of the rice cultivars and HCN was in the range of 85.5 to 99.5%. The RS extracted from the rice cultivars and HCN showed significant difference in the apparent amylose content (AAC), ranging between 31.83 to 40.68% for rice and 45.79% for HCN. Water absorption capacity (WAC), swelling and solubility index of RS ranged from 112-133.9%, 5.28-7.25 g/g and 0.033-0.044 g/g, respectively. The rice RS granules were polyhedral and irregular shaped with granular length in the range of 4.8-5.9 µm. The HCN RS granule morphology showed smooth surfaced, round, elliptical, irregular and oval shapes with average granular length of 21 µm. Pasting behavior also varied significantly between rice RS and HCN RS with later showing the lower values of pasting properties. Thermal properties (T, T T) and ΔH gel also varied considerably between the rice RS and HCN RS, wherein the highest values for peak gelatinization temperature and gelatinization enthalpy were seen for CH-1039. X-ray diffraction pattern of rice RS and HCN RS showed the characteristic A type of pattern in consonance with cereal starches.
本研究从五种喜马拉雅水稻品种和印度马栗中提取抗性淀粉（RS），使用猪胰蛋白酶酶法，随后对其理化性质、结构和功能特性进行了表征。体外消化试验表明，水稻品种和印度马栗的 RS 含量在 85.5%至 99.5%之间。从水稻品种和印度马栗中提取的 RS 在表观直链淀粉含量（AAC）方面存在显著差异，范围在 31.83%至 40.68%之间，而印度马栗为 45.79%。RS 的水吸收能力（WAC）、膨胀度和溶解度指数分别为 112-133.9%、5.28-7.25 g/g 和 0.033-0.044 g/g。水稻 RS 颗粒呈多面体形和不规则形状，颗粒长度在 4.8-5.9 µm 之间。印度马栗 RS 颗粒形态呈光滑表面、圆形、椭圆形、不规则和椭圆形，平均颗粒长度为 21 µm。糊化特性也在水稻 RS 和印度马栗 RS 之间存在显著差异，后者表现出较低的糊化特性值。热特性（T、T T）和ΔH 凝胶也在水稻 RS 和印度马栗 RS 之间有很大差异，其中 CH-1039 的峰值糊化温度和糊化焓值最高。水稻 RS 和印度马栗 RS 的 X 射线衍射图谱显示出与谷物淀粉一致的特征 A 型图谱。
