Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.
Vietnamese-German University, Thu Dau Mot, Binh Duong, Vietnam.
Sci Rep. 2020 Mar 5;10(1):4081. doi: 10.1038/s41598-020-60806-9.
This paper presents a highly sensitive closed loop enclosed split ring biosensor operating in microwave frequencies for measuring blood glucose levels in the human body. The proposed microwave glucose biosensor, working on the principle of high field confinement and concentrated energy, has been tested using both in-vitro and in-vivo methods. This principle allows the sensor to concentrate energy at the surface which results in improved accuracy of measurements. For in-vitro measurements, the biosensor has been tested using de-ionized water glucose solutions of different concentrations. The miniaturized micrometer scale biosensor is fabricated over a thin Si-substrate using photolithographic technique. The biosensor has been designed in a way to operate at desired microwave frequencies. Highly confined fields and concentrated energy inside the closed loop line containing the split ring resonators are responsible for the sensitivity enhancement. This new biosensor has obtained a high sensitivity of 82 MHz/mgmL within the clinical diabetic range during in-vivo testing over the human body. In addition, the subjects (undergoing experiments) steady state has been continuously monitored throughout the experiment which helps in improving the accuracy of the results. The proposed biosensor has further obtained a low detection limit of <0.05 wt.% and can be useful for continuous non-invasive blood glucose monitoring.
本文提出了一种工作在微波频率下的高灵敏度闭环封闭环形生物传感器，用于测量人体中的血糖水平。所提出的微波葡萄糖生物传感器基于高场限制和集中能量的原理，已经通过体外和体内方法进行了测试。该原理允许传感器将能量集中在表面，从而提高测量的准确性。对于体外测量，该生物传感器已经使用不同浓度的去离子水葡萄糖溶液进行了测试。微型化的微尺度生物传感器使用光刻技术在薄 Si 衬底上制造。该生物传感器的设计方式使其能够在所需的微波频率下工作。在包含分裂环谐振器的闭环线内，高度限制的场和集中的能量负责提高灵敏度。在对人体进行的体内测试中，这种新的生物传感器在临床糖尿病范围内获得了 82 MHz/mgmL 的高灵敏度。此外，实验对象（正在进行实验）的稳态在整个实验过程中持续监测，这有助于提高结果的准确性。所提出的生物传感器进一步获得了<0.05wt.%的低检测限，可用于连续的非侵入性血糖监测。
