Manna Subhankar, Dey Aditi, Majumdar Rakhi, Bag Braja Gopal, Ghosh Chandradipa, Roy Somenath
Immunology and Microbiology Laboratory, Department of Human Physiology with Community Health, Vidyasagar University, Midnapore 721 102, West Bengal, India.
Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore, 721102, West Bengal, India.
Heliyon. 2020 Feb 29;6(2):e03456. doi: 10.1016/j.heliyon.2020.e03456. eCollection 2020 Feb.
Arjunolic acid (AA) a plant derived pentacyclic triterpenoid which showed effective anticancer activity against MCF-7 and HeLa cells as well as no significant toxic effect was observed against normal lymphocytes. In the current study the self assemble property of arjunolic acid gives an extra emphasis on anticancer activity which was proved by several fluorescence studies like ROS generation, EtBr/AO and DAPI staining. At a selected dose of 50μg/ml AA disrupt the redox balance inside the cancer cells by producing reactive oxygen species. The apoptotic event was mediated by two key regulator proteins TNF-α and NF-κß which was proved here. The increment of the pro-inflammatory cytokines indicates the ROS mediated pathway of cancer cell apoptosis.
阿朱诺酸（AA）是一种植物来源的五环三萜类化合物，对MCF-7和HeLa细胞显示出有效的抗癌活性，并且对正常淋巴细胞未观察到明显的毒性作用。在当前研究中，阿朱诺酸的自组装特性进一步强调了其抗癌活性，这已通过多项荧光研究得到证实，如活性氧生成、溴化乙锭/吖啶橙和4',6-二脒基-2-苯基吲哚染色。在选定的50μg/ml剂量下，阿朱诺酸通过产生活性氧破坏癌细胞内的氧化还原平衡。在此证明，凋亡事件由两种关键调节蛋白肿瘤坏死因子-α（TNF-α）和核因子κB（NF-κB）介导。促炎细胞因子的增加表明癌细胞凋亡的活性氧介导途径。
