Mao Deqian, Chen Fengge, Wang Rui, Bai Ping, Zhang Yanhong, Zhao Wei, Chen Jing, Yang Lichen, Yang Xiaoguang, Li Min
Key Laboratory of Trace Element Nutrition, National Health Commission; National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention, Beijing, China.
Shijiazhuang Center for Disease Control and Prevention, Shijiazhuang, Hebei, China.
J Nutr. 2020 May 1;150(5):1208-1213. doi: 10.1093/jn/nxaa031.
Due to a lack of research data on the protein requirements of the elderly in China, the estimated average requirement (EAR) and the recommended nutrient intake (RNI) of protein in the elderly remain the same as those in young and middle-aged people at 0.98 g/(kg·d).
The objective of this study was to determine the protein requirements of healthy Chinese adults >65y old through use of the indicator amino acid oxidation (IAAO) method.
Seven healthy adult men and 7 healthy adult women participated in the study, with protein intakes ranging from 0.3 to 1.8 g/(kg·d). The diets were isocaloric and provided energy at a 1.5 resting energy expenditure. Protein was given based on the lactalbumin. Phenylalanine and tyrosine were added to protein doses of 0.3-1.5 g/kg according to the highest dose of protein content [1.8 g/(kg·d)]. Phenylalanine and tyrosine concentrations were kept constant at each protein dose. The mean protein requirement was determined by applying a nonlinear mixed-effects model analysis to the F13CO2, which identified a breakpoint in F13CO2 in response to graded amounts of protein. This trial was registered with the Chinese clinical trial registry as ChiCTR-BOC-17010930.
Protein EAR and RNI for healthy elderly Chinese adults were determined to be 0.91 and 1.17 g/(kg·d), respectively, based on the indicator amino acid oxidation technique.
The estimates of protein requirements for Chinese adults >65 y in the present study are 3.4% and 19.4% higher than the current estimated requirements, 0.88 g/(kg·d) for EAR and 0.98 g/(kg·d) for RNI.
由于中国缺乏关于老年人蛋白质需求的研究数据，老年人蛋白质的估计平均需求量（EAR）和推荐营养素摄入量（RNI）仍与中青年人群相同，为0.98克/（千克·天）。
本研究旨在通过使用指示氨基酸氧化（IAAO）方法确定65岁以上健康中国成年人的蛋白质需求量。
7名健康成年男性和7名健康成年女性参与了本研究，蛋白质摄入量范围为0.3至1.8克/（千克·天）。饮食为等热量饮食，提供的能量为静息能量消耗的1.5倍。蛋白质以乳白蛋白为基础给予。根据最高蛋白质含量剂量[1.8克/（千克·天）]，在0.3 - 1.5克/千克的蛋白质剂量中添加苯丙氨酸和酪氨酸。在每个蛋白质剂量下，苯丙氨酸和酪氨酸的浓度保持恒定。通过对F13CO2应用非线性混合效应模型分析来确定平均蛋白质需求量，该分析确定了F13CO2中响应不同蛋白质剂量的一个断点。本试验已在中国临床试验注册中心注册，注册号为ChiCTR - BOC - 17010930。
基于指示氨基酸氧化技术，确定健康中国老年成年人的蛋白质EAR和RNI分别为0.91和1.17克/（千克·天）。
本研究中65岁以上中国成年人的蛋白质需求估计值比当前估计需求量分别高出3.4%和19.4%，当前EAR估计值为0.88克/（千克·天），RNI为0.98克/（千克·天）。
