佛罗里达海牛（西印度海牛指名亚种，海牛目）的完整线粒体基因组

Complete mitochondrial genome of the Florida manatee (Trichechus manatus latirotris, Sirenia).

作者信息

Vilaça Sibelle T, Santos Fabricio R

机构信息

Trent University, Environmental and Life Sciences Graduate Program, Peterborough, Ontario, Canada.

Universidade Federal de Minas Gerais, Departamento de Genética, Ecologia e Evolução, Laboratório de Biodiversidade e Evolução Molecular, Belo Horizonte, MG, Brasil.

出版信息

Genet Mol Biol. 2020 Feb 3;42(4):e20190210. doi: 10.1590/1678-4685-GMB-2019-0210. eCollection 2020.

DOI:10.1590/1678-4685-GMB-2019-0210

PMID:32142097

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7197981/

Abstract

The Florida manatee (Trichechus manatus latirostris) is an endangered subspecies of the West Indian manatee (T. manatus), which inhabits inland and marine waters of southeastern United States. In this study, we assembled the mitochondrial genome (mtDNA) of the Florida manatee from whole genome shotgun reads. As a result, we show that the currently annotated T. manatus mtDNA belongs to a different species, the Amazonian manatee (T. inunguis). The newly assembled Florida manatee mtDNA is 16,881 bp in length, with 13 protein-coding genes, two ribosomal RNAs (rRNAs), 22 transfer RNAs (tRNAs) and one non-coding control region (D-loop). Phylogenetic analysis based on the control region indicates the newly assembled mtDNA is haplotype A01, characteristic of T. m. latirostris, while the current mtDNA associated with the Florida manatee genome assembly has a Ti02 haplotype that is found in Amazonian manatees and hybrids.

摘要

佛罗里达海牛（Trichechus manatus latirostris）是西印度海牛（T. manatus）的一个濒危亚种，栖息于美国东南部的内陆和沿海水域。在本研究中，我们从全基因组鸟枪法测序读段中组装出了佛罗里达海牛的线粒体基因组（mtDNA）。结果表明，目前注释的T. manatus mtDNA属于一个不同的物种——亚马逊海牛（T. inunguis）。新组装的佛罗里达海牛mtDNA长度为16,881 bp，包含13个蛋白质编码基因、两个核糖体RNA（rRNA）、22个转运RNA（tRNA）和一个非编码控制区（D环）。基于控制区的系统发育分析表明，新组装的mtDNA是A01单倍型，这是T. m. latirostris的特征，而当前与佛罗里达海牛基因组组装相关的mtDNA具有在亚马逊海牛和杂交种中发现的Ti02单倍型。