Beijing National Laboratory for Molecular Sciences; Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, P.R. China.
School of Chemical Sciences, University of Chinese Academy of Sciences, Beijing 100049, P.R. China.
Anal Chem. 2020 Mar 17;92(6):4445-4450. doi: 10.1021/acs.analchem.9b05393. Epub 2020 Mar 6.
To monitor d-glucose (Glu) in complex aqueous media with a high specificity, a conceptually new "selective capture and controllable detection" nanoreactor was explored. We designed and synthesized poly maleic anhydride-styrene--isopropylacrylamide-(4-aminophenyl) boronic acid [P(MAn-St-NIPAm-PBA)] to fabricate the nanoreactor. On the surface of the self-assembled, micelle-based nanoreactor, the stereo precise placement PBA provided a recognition unit in the block copolymer structure to boost the selective capture of Glu over other saccharides. P(MAn-St-NIPAm) served as the thermal sensitive moiety of the nanoreactor, which embedded with glucose oxidase and myoglobin-based catalyst in order to realize the controllable enzymolysis of Glu through temperature alteration. Once the nanoreactor was mixed with Glu, an obvious change in the UV-visible intensity of quinine produced in the multienzymolysis was observed. Glu in the rat microdialysates of brain ischemia was successfully monitored by the nanoreactor method, demonstrating the feasibility of constructing high-specificity nanoreactors for cerebral system applications.
为了在复杂的水相介质中具有高特异性地监测 d-葡萄糖（Glu），探索了一种概念新颖的“选择性捕获和可控检测”纳米反应器。我们设计并合成了马来酸酐-苯乙烯-异丙基丙烯酰胺-（4-氨基苯基）硼酸[P（MAn-St-NIPAm-PBA）]来制备纳米反应器。在自组装的基于胶束的纳米反应器表面上，立体精确放置的 PBA 在嵌段共聚物结构中提供了一个识别单元，以增强 Glu 与其他糖的选择性捕获。P（MAn-St-NIPAm）作为纳米反应器的热敏部分，其中嵌入了葡萄糖氧化酶和基于肌红蛋白的催化剂，以通过温度变化实现 Glu 的可控酶解。一旦纳米反应器与 Glu 混合，就可以观察到多酶解过程中产生的奎宁的紫外可见强度发生明显变化。通过纳米反应器方法成功监测了脑缺血大鼠微透析液中的 Glu，证明了构建用于脑系统应用的高特异性纳米反应器的可行性。
