University of Warmia and Mazury in Olsztyn, Department of Environment Engineering, Warszawska St. 117a, Olsztyn 10-719, Poland.
Bialystok University of Technology, Department of Environmental Engineering Technology, Wiejska St. 45E, Bialystok 15-351, Poland.
Sci Total Environ. 2020 Jun 10;720:137377. doi: 10.1016/j.scitotenv.2020.137377. Epub 2020 Feb 19.
Wastewater from households wastewater treatment plants (HWWTP) is discharged to the ground or to the surface waters. Special consideration should be given to the improvement of HWWTP effectiveness, particularly in relation to nutrients. The addition of biodegradable carbon sources to biofilm reactor, can enhance microbial activity but may also lead to filling clogging. The study aimed to compare 3 different organic substrates: acetic acid (commonly applied)and two untypical - citric acid and waste beer, under the same operational conditions in a post-denitrification biofilm reactor. The study investigated the impact of a type of organic substrate, low pH and time on: (1) biofilm growth, (2) the characteristics of extracellular polymeric substances (EPS), (3) the kinetics of nutrients removal and (4) reactor clogging. Results were referred to (5) the effectiveness of nutrients removal. The study demonstrated that low pH assured the development of a thinbiofilm. Citric acid ensured the lowest biomass volume, being by 53% lower than in the reactor with acetic acid and by as much as 61% lower than in the reactor with waste beer. The soluble EPS fraction prevailed in the total EPS in all reactors. The content of the tightly bound EPS fraction ranged from 26.93% (citric acid) to 36.32% (waste beer). Investigations showed also a high ratio of exoproteins to polysaccharide in all fractions, which indicated a significant role of proteins in developing a highly-proliferating biofilm. The treated wastewater met requirements of Polish regulations concerning COD and nitrogen concentrations.
家庭废水处理厂（HWWTP）的废水被排放到地面或地表水中。应特别注意提高 HWWTP 的效率，特别是与营养物有关的效率。在生物膜反应器中添加可生物降解的碳源可以增强微生物的活性，但也可能导致填充堵塞。本研究旨在比较 3 种不同的有机基质：乙酸（常用）和两种非典型的 - 柠檬酸和废啤酒，在相同的操作条件下，在后反硝化生物膜反应器中。该研究调查了有机基质的类型、低 pH 值和时间对以下方面的影响：（1）生物膜生长，（2）细胞外聚合物物质（EPS）的特性，（3）营养物去除动力学，（4）反应器堵塞。结果与（5）营养物去除的有效性有关。该研究表明，低 pH 值保证了薄生物膜的发展。柠檬酸确保了最低的生物量体积，比在含有乙酸的反应器中低 53%，比在含有废啤酒的反应器中低 61%。在所有反应器中，可溶性 EPS 分数在总 EPS 中占主导地位。紧密结合的 EPS 分数的含量范围从 26.93%（柠檬酸）到 36.32%（废啤酒）。研究还表明，所有分数中的外蛋白与多糖的比例都很高，这表明蛋白质在形成高度增殖的生物膜方面发挥了重要作用。处理后的废水符合波兰法规关于 COD 和氮浓度的要求。
