Tiemann Markus, Samoilova Vera, Atiakshin Dmitri, Buchwalow Igor
Institute for Hematopathology, Hamburg, Germany.
Research Institute of Experimental Biology and Medicine, Burdenko Voronezh State Medical University, Voronezh, Russia.
BMC Res Notes. 2020 Mar 7;13(1):139. doi: 10.1186/s13104-020-04975-w.
Programmed death-1 (PD-1) and its ligand PD-L1 are now used as predictive biomarkers to guide clinical decisions. Precise characterization of PD-L1-positive cells may contribute to our knowledge of which patients derive benefit from the PD-L1 blockade therapy.
To address this issue, we performed immunophenotyping of PD-L1-positive cells in Hodgkin lymphoma and in angioimmunoblastic T cell lymphoma (AITL) employing multiple immunofluorescent immunolabeling. We found that PD-L1-positive cells and PD-1-positive cells both in Hodgkin lymphoma and in AITL belong to two completely different cell lineages. In both lymphomas, PD-1 was found exclusively in T-lymphocytes, whereas PD-L1 was revealed in the tumor microenvironment cells including macrophages. PD-L1 was also detected in CD30-positive cells in Hodgkin lymphoma but not in AITL. The marker of B-cell lineage, CD20, was not detectable in PD-L1-positive cells both in AITL and in Hodgkin. Our study highlights the importance of comprehensive assessment of PD-1/PD-L1 regulatory pathways for employing PD-L1 as a predictive biomarker in clinical practice. PD-L1-antibody therapy is proven in Hodgkin lymphoma. Comparative immunophenotyping of the PD-1/PD-L1 axis provides a support for attempts to prove this principle also for AITL.
程序性死亡蛋白1（PD-1）及其配体PD-L1现被用作预测生物标志物以指导临床决策。对PD-L1阳性细胞进行精确表征可能有助于我们了解哪些患者能从PD-L1阻断治疗中获益。
为解决这一问题，我们采用多重免疫荧光免疫标记技术对霍奇金淋巴瘤和血管免疫母细胞性T细胞淋巴瘤（AITL）中的PD-L1阳性细胞进行了免疫表型分析。我们发现，霍奇金淋巴瘤和AITL中的PD-L1阳性细胞和PD-1阳性细胞均属于两个完全不同的细胞谱系。在这两种淋巴瘤中，PD-1仅在T淋巴细胞中发现，而PD-L1则在包括巨噬细胞在内的肿瘤微环境细胞中显示。在霍奇金淋巴瘤的CD30阳性细胞中也检测到了PD-L1，但在AITL中未检测到。在AITL和霍奇金淋巴瘤的PD-L1阳性细胞中均未检测到B细胞谱系标志物CD20。我们的研究强调了全面评估PD-1/PD-L1调节通路对于在临床实践中采用PD-L1作为预测生物标志物的重要性。PD-L1抗体治疗在霍奇金淋巴瘤中已得到证实。对PD-1/PD-L1轴进行比较性免疫表型分析为尝试在AITL中也证明这一原理提供了支持。
