Institut Curie, PSL Research University, CNRS, Sorbonne Université, Nuclear Dynamics, Paris, France; Ecole Normale Supérieure, PSL Research University, CNRS, Sorbonne Université, Laboratoire de Physique, Paris, France.
Ecole Normale Supérieure, PSL Research University, CNRS, Sorbonne Université, Laboratoire de Physique, Paris, France.
Curr Top Dev Biol. 2020;137:119-142. doi: 10.1016/bs.ctdb.2019.12.002. Epub 2020 Jan 17.
The regulation of the hunchback promoter expression by the maternal Bicoid gradient has been studied as a model system in development for many years. Yet, at the level of quantitative agreement between data and theoretical models, even the first step of this regulation, transcription, continues to be challenging. This situation is slowly progressing, thanks to quantitative live-imaging techniques coupled to advanced statistical data analysis and modeling. Here, we outline the current state of our knowledge of this apparently "simple" step, highlighting the newly appreciated role of bursty transcription dynamics and its regulation.
多年来，人们一直将 hunchback 启动子表达受母体 Bicoid 浓度梯度的调控作为发育的模型系统进行研究。然而，在数据与理论模型定量吻合的层面上，即使是这一调控的第一步——转录，仍然具有挑战性。这种情况正在逐步改善，这要归功于定量活体成像技术与先进的统计数据分析和建模的结合。在这里，我们概述了目前对这一看似“简单”步骤的认识，强调了新发现的突发转录动力学及其调控的作用。
