Departamento de Biotecnología, Universidad Autónoma Metropolitana, Iztapalapa, P.A. 55-535, 09340, Mexico City, Mexico.
Facultad de Química, Universidad Nacional Autónoma de México, Mexico City, Mexico.
Bioprocess Biosyst Eng. 2020 Jul;43(7):1219-1230. doi: 10.1007/s00449-020-02316-6. Epub 2020 Mar 6.
The bioconversion process of bioactive naringenin by whole-cells of Yarrowia lipolytica 2.2ab for the production of increased value-added compounds is successfully achieved in surface and liquid cultures. This approach is an alternative to the commercial production of these bioactive compounds from vegetable sources, which are limited due to their low concentrations and the complexity of the purification processes. The experimentation rendered seven value-added compounds in both surface and liquid bioconversion cultures. Some of the compounds produced have not been previously reported as products from the bioconversion processes, such as the case of ampelopsin. Biosynthetic pathways were suggested for the naringenin bioconversion using whole-cells of Y. lipolytica 2.2ab. Finally, the extracts obtained from the naringenin bioconversion in liquid cultures showed higher percentage of inhibition of DPPH· and ABTS· radicals up to 32.88 and 2.08 times, respectively, compared to commercial naringenin.
利用酿酒酵母 2.2ab 全细胞将具有生物活性的柚皮素进行生物转化，成功实现在表面和液体培养中生产高附加值化合物。与从蔬菜来源商业生产这些生物活性化合物相比，这种方法是一种替代方法，因为蔬菜来源的生物活性化合物浓度低，且纯化过程复杂。实验在表面和液体生物转化培养物中得到了七种高附加值化合物。一些所产生的化合物以前并未被报道为生物转化过程的产物，例如安贝司汀的情况。利用酿酒酵母 2.2ab 全细胞，提出了柚皮素生物转化的生物合成途径。最后，与商业柚皮素相比，液体培养物中柚皮素生物转化得到的提取物对 DPPH·和 ABTS·自由基的抑制率分别高达 32.88 和 2.08 倍。
