Hernández-Guzmán Christian, Hernández-Montiel Luis G, Velázquez-Lizarraga Adrian E, Ríos-González Leopoldo J, Huerta-Ochoa Sergio, Cervantes-Güicho Vianey de J, Morales-Martínez Thelma K, Mejía-Ruíz Claudio H, Reyes Ana G
Unidad Iztapalapa, Departamento de Biotecnología, Universidad Autónoma Metropolitana, México City 02128, Mexico.
Centro de Investigaciones Biológicas del Noroeste, Programa de Agricultura en Zonas Áridas, Av. Instituto Politécnico Nacional 195, Playa Palo de Santa Rita Sur, La Paz 23096, Mexico.
Plants (Basel). 2024 Sep 20;13(18):2629. doi: 10.3390/plants13182629.
This study explores the bioconversion of diosgenin by , focusing on enhancing the antioxidant, antifungal, and herbicidal activities of the resulting extracts. The bioconversion process, involving glycosylation and hydroxylation, produced significant amounts of protodioscin and soyasaponin I. The extracts showed superior antioxidant activity, with up to 97.02% inhibition of ABTS· radicals and 33.30% inhibition of DPPH· radicals at 1000 mg L of diosgenin. Antifungal assays revealed strong inhibitory effects against , sp., and , with maximum inhibition rates of 67.34%, 35.63%, and 65.53%, respectively. Additionally, the herbicidal activity of the bioconverted extracts was comparable to commercial herbicides, achieving 100% inhibition of seed germination in both monocotyledonous and dicotyledonous plants. These findings suggest that the -mediated bioconversion of diosgenin could provide a sustainable and eco-friendly alternative for developing natural biofungicides and bioherbicides.
本研究探索了[具体微生物]对薯蓣皂苷元的生物转化，重点在于提高所得提取物的抗氧化、抗真菌和除草活性。该生物转化过程涉及糖基化和羟基化，产生了大量的原薯蓣皂苷和大豆皂苷I。提取物表现出优异的抗氧化活性，在薯蓣皂苷元浓度为1000 mg/L时，对ABTS·自由基的抑制率高达97.02%，对DPPH·自由基的抑制率为33.30%。抗真菌试验表明，对[具体真菌1]、[具体真菌2]菌和[具体真菌3]有很强的抑制作用，最大抑制率分别为67.34%、35.63%和65.53%。此外，生物转化提取物的除草活性与商业除草剂相当，对单子叶植物和双子叶植物种子萌发的抑制率均达到100%。这些发现表明，[具体微生物]介导的薯蓣皂苷元生物转化可为开发天然生物杀菌剂和生物除草剂提供一种可持续且环保的替代方案。
