Animal Science and Technology, Nanjing Agricultural University, No.1 Weigang, Nanjing, 210095, Jiangsu, People's Republic of China.
Animal Production Research Institute, Agriculture Research Centre, Dokki, Giza, 12618, Egypt.
Environ Sci Pollut Res Int. 2020 May;27(14):16865-16875. doi: 10.1007/s11356-020-08269-3. Epub 2020 Mar 6.
Glyphosate is the active component of several commercial formulations as in Roundup®. The present study was investigated the toxic effects of pure glyphosate or Roundup® on the liver and small intestine of chick embryos. On day 6, a total of 180 fertile eggs injected with deionized water (control group), 10 mg pure glyphosate, or 10 mg of the active ingredient glyphosate in Roundup®/kg egg mass. The results showed an increase in relative weights of the liver in embryos that treated with Roundup®. Furthermore, oxidative stress was observed in the embryos treated with glyphosate or Roundup®, increased total superoxide dismutase, and content of malondialdehyde in the liver and intestine; moreover, decrease of glutathione peroxidase in the liver with increased in the intestine compared with the control. Besides, glutamic-pyruvic transaminase was increased in Roundup® group compared with other groups. Moreover, histopathological alterations in the liver and intestine tissues were observed in treated groups. Suppression of hepatic CYP1A2, CYP1A4, CYP1B1, and MDR1 mRNA expression after exposed to Roundup®. Furthermore, inhibition of CYP1A4 in the duodenum, CYP1A4, and MRP2 in the jejunum in embryos exposed to glyphosate or Roundup®. In addition, glyphosate treatment caused an increase of CYP3A5, CYP1C1, and IFNY mRNA expression in the jejunum and CYP1A2 expression in the ileum, while IFN-Y gene increase in embryos treated with Roundup®. In conclusion, in ovo exposure to glyphosate caused histopathological alterations and induced oxidative stress in the liver and small intestines. Moreover, the expression of cytochrome P450, MDR1, and MRP2 transporters was also modulated in the liver and small intestines for chick embryos.
草甘膦是多种商业制剂（如 Roundup®）中的有效成分。本研究旨在研究纯草甘膦或 Roundup®对鸡胚肝脏和小肠的毒性作用。在第 6 天，共向 180 枚受精蛋注射去离子水（对照组）、10mg 纯草甘膦或 10mg 草甘膦在 Roundup®/kg 卵质量中的活性成分。结果表明，用 Roundup®处理的胚胎肝脏相对重量增加。此外，用草甘膦或 Roundup®处理的胚胎中观察到氧化应激，肝脏和肠道中超氧化物歧化酶总量增加，丙二醛含量增加；此外，与对照组相比，肝脏谷胱甘肽过氧化物酶减少，肠道增加。此外，与其他组相比，Roundup®组谷氨酸-丙酮酸转氨酶增加。此外，在处理组中观察到肝脏和肠道组织的组织病理学改变。暴露于 Roundup®后，肝脏 CYP1A2、CYP1A4、CYP1B1 和 MDR1mRNA 表达受到抑制。此外，草甘膦或 Roundup®暴露后，十二指肠中 CYP1A4 和 MRP2 以及空肠中 CYP1A4 被抑制。此外，草甘膦处理导致空肠中 CYP3A5、CYP1C1 和 IFNYmRNA 表达增加，回肠中 CYP1A2 表达增加，而 Roundup®处理的胚胎中 IFN-Y 基因增加。总之，鸡胚体内暴露于草甘膦导致肝脏和小肠组织发生组织病理学改变并诱导氧化应激。此外，鸡胚肝脏和小肠中细胞色素 P450、MDR1 和 MRP2 转运体的表达也发生了调节。
